Dekalb Genetics Corporation

Other

Monsanto Technology LLC

Michael A Stephens

Michael Spencer

Gregory R Heck

David A Walters

Paul C Anderson

Albert P Kausch

Emil M Orozco

Rita Mumm

Paul S Chomet

Michael A Hall

William J Gordon-Kamm

Catherine J Mackey

Peter M Orr

Donald S Walters

Christopher E Flick

David McElroy

J Jefferson Gwyn

Michael T Mann

Peggy G Lemaux

Thomas R Adams

T Michael Spencer

James V O'Brien

Richard J Daines

Nancy G Willetts

Lucille B Laccetti

Thomas B Rice

William G Start

Sheryl A Chambers

Mary L Mangano

Clayton S Vetsch

Susan J Zachwieja

Kimberly Glassman

Paul CC Feng

Charles Lester Armstrong

Martha-Ann Post-Beittenmiller

Thomas J Peters

This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs thebar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof

Methods and compositions relating to glyphosate resistant maize plants, including the GA21, GG25, GJ11 and FI117 transformation events, are disclosed. Also disclosed are methods of using herbicide resistance transformation events in plant breeding procedures. The invention further includes methods of ensuring plant seed purity.

Method for plant breeding

This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

The invention is directed to a method of breeding maize using female plants having a transgene encoding an EPSPS enzyme where the female plants are female reproductively tolerant to treatment with glyphosate but can be made male sterile upon treatment with glyphosate.

Method of plant breeding

A protecter for a riser connected to an underground water pipe and sprinkler head attached at a top portion thereof includes an elongate hollow body member having an upper portion with an upper opening and a lower portion with a lower opening, each opening being defined by a rim on respective upper and lower portion. The body defines an interior space for housing a riser and sprinkler head therein and has a lower portion including a pair of slots formed therein for fitting the body over an underground water pipe, and an upper portion further including a concave hood member extending laterally outwardly and downwardly of the rim of the upper opening and defining a cavity. The hood member has a lower edge portion for engaging a ground surface to limit downward travel of the protecter. The slots have an open lower portion and an arcuate upper portion for engaging the upper surface of an underground water pipe. The protecter also includes a plurality of spaced flanges extending beneath the hood member and engaged with the body to inhibit rotative motion of the hood member by positioning the flanges into a ground surface. The lower edge portion is located in a plane beneath an underground water pipe.

Sprinkler head and riser protector

Growing community of inventors

East Lyme, CT, United States of America

Michael A Stephens