New England Biolabs, Inc.

Michael A Dalton

Huimin Kong

Lauren Sears Higgins

Shuang-Yong Xu

Geoffrey G Wilson

Keith D Lunnen

Rebecca B Kucera

Ira Schildkraut

Jack Benner

Andrew Dore

Rebecca Kucera

The present invention relates to recombinant DNA that encodes the TspRI restriction endonuclease as well as TspRI methylase, expression of TspRI restriction endonuclease and TspRI methylase in cells containing the recombinant DNA.

Method for cloning and expression of TspRI restriction endonuclease and TspRI methylase in E. coli

The present invention relates to the recombinant DNA which encodes the SwaI restriction endonuclease, modification methylase, and the production of SwaI restriction endonuclease from the recombinant DNA. Related expression vectors, pHKUV5 which features a strong, constitutive UV5 promoter without the Lac repressor binding site and pHKT7 which contains a powerful controllable T7 promoter and a low copy number origin of replication, are also disclosed.

Method for cloning and producing the SwaI restriction endonuclease

The present invention relates to recombinant DNA which encodes a novel nicking endonuclease, N.BstNBI, and the production of N.BstNBI restriction endonuclease from the recombinant DNA utilizing PleI modification methylase. Related expression vectors, as well as the application of N.BstNBI in non-thio strand displacement amplification, is disclosed also.

Cloning and producing the N.BstNBI nicking endonuclease

The present invention relates to the recombinant DNA which encodes the DraIII restriction endonuclease modification methylase, and the production of DraIII restriction endonuclease from the recombinant DNA. Related expression vectors, pHKUV5 vector which features a strong, constitutive UV5 promoter without the Lac repressor binding site and pHKT7 vector which contains a powerful controllable T7 promoter and a low copy number origin of replication, are also disclosed.

Method for cloning and producing the DraIII restriction endonuclease

The present invention relates to the recombinant DNA which encodes the SgrAI restriction endonuclease and the MspI modification methylase, and the production of SgrAI restriction endonuclease from the recombinant DNA.

Method for cloning and producing the SgrAI restriction endonuclease

A recombinant restriction endonuclease from Anabaena variabilis is provided, as well as the isolated gene which encodes it and methods for the production of the recombinant AvaI restriction endonuclease. An isolated gene encoding a modification methylase from A. variabilis is also provided.

Growing community of inventors

Manchester, MA, United States of America

Michael A Dalton