Invitrogen Corporation

Life Technologies Corporation

Becton Dickinson and Company Limited

Michael A Brasch

James L Hartley

Gary F Temple

David Cheo

John J Carrino

Knut R Madden

Louis Leong

James Fan

Timothy M Wiles

Martin Anthony Gleeson

Patrick Shawn Beaty

Jonathan D Chesnut

Song Shi

Ming-hsiung Yeh

Mei Yang-Woytowitz

Donna K Fox

Dwight Livingston

James G Nadeau

Ming-Hsiung Yeh

Brent Ronald Pohl

Charles C Yu

Kerry Lynn Smith

Ammon David Lentz

William Alan Fox

Raphael Rodolphe Marcelpoil

Alexander George Lastovich

Richard L Moore

William Kevin Carpenter

Kevin Bailey

Joshua Herr

Owen Lewis Joyce

Kristin Weidemaier

Ben Turng

Brian Reuben Langhoff

Eric Fallows

Qihua Xu

Devon R Byrd

Liping Feng

Julie L Rosales

David S Sebba

Trienko Marten Van Der Kaap

Richard Abbot

Devon R N Byrd

Gary F Hershner

Jon E Salomon

Xiaofei Chang

Suneil Hosmane

Patrick R Murray

Eric Ursprung

David Polley

Jennifer Singelyn

James Y Zhou

Alexander Adam Papp

Curtis M Gosnell

Cristian Clavijo

Jonathan Chestnut

Michael Bois

Devon Rn Byrd

Vikram Patel

Gerard Lotz

Sean Tucker

Bas Nieuwenhuis

Gray F Temple

Sean Connell

Martin A G Gleeson

Shirley Ng

Timothy Mark Wiles

Meghan Wolfgang

A system and method with increased sensitivity to microorganism growth. The system includes signal processing electronic circuit connected to a consumable or vessel through two or more electrodes that fully penetrate the vessel and are in contact with the fluid contents. The electronic circuit is configured to detect a component of the total impedance of the sample, specifically the “out-of-phase” or imaginary reactance component, which has a sensitive response to organism growth in a frequency-dependent manner. The system detects changes in both the composition of charged molecules in the liquid matrix and the number of microorganisms based on monitoring the sample for change in this parameter. This results in a 5-70% reduction in time-to-detection (TTD). The system and method detect organisms in a plurality of vessel shapes, volumes, and matrix (or media) formats. The electrodes are fully immersed in a continuous body of liquid sample. The distance between electrodes may be adjusted or tuned to fit the needs of the vessel. The voltage inputs can also be adjusted to allow proper detection of the contents within the vessel.

Reactance and capacitance sensing platform for detecting microorganisms

A container for culturing a blood sample. The container has a reservoir that is no larger than about 40 ml in volume with culture media therein varying in amount by volume 0.5 ml to about 20 ml. The container is adapted to receive a blood sample drawn from a patient, wherein the blood volume is about 1 ml to about 20 ml. In some embodiments the ratio of blood volume to culture media volume is about 2:1 to about 1:2 and the volume of blood does not exceed about 10 ml. In some embodiments, the media is lytic media. A method for using the container to culture a blood sample is also contemplated. In such method, the container is inoculated with the blood sample. In certain embodiments, the volume of the blood sample does not exceed 10 mls.

Blood culture system with low media volume

An apparatus and associated methods of use for a controlled combination of reagents is disclosed. The apparatus includes a vessel, a vessel insert, and a cap element. The vesselhas a body portionfor receiving a biological sample. The vessel insertreceives at least one reagent therein. Preferably, the vessel insertis received in a portionof the vessel. The cap elementis attached to the vesselto secure the vessel insertin the vessel. During use, the vessel insertis adapted to release its contents when the biological sample is introduced into the body portionof the vesselupon application of an intermixing force to the vessel insert. A variety of intermixing forces may be applied, depending upon the embodiment of the present invention and its associated methods of use.

Blood culture bottles with mechanisms for controlled release of substances into culture media

A system and method for developing applications (Apps) for automated assessment and analysis of processed biological samples. Such samples are obtained, combined with nutrient media and incubated. The incubated samples are imaged and the image information is classified according to predetermined criteria. The classified image information is then evaluated according to Apps derived from classified historical image information in a data base. The classified historical image information is compared with the classified image information to provide guidance on further processing of the biological sample through Apps tailored to process provide sample process guidance tailored to the classifications assigned to the image information.

Application development environment for biological sample assessment processing

A device for collecting a biological sample from a semi-solid surface. The device has a shaft with a proximate end and a distal end and a tip integrated with the shaft at the proximate end. The tip has a surface adapted to collect microorganisms thereon or release microorganism from, or both, wherein the adapted surface comprises at least one feature of a recess or extension to increase surface area of the tip and collect microorganisms thereon. Examples of such features include microfeatures with dimensions of about 1000 &#x3bc;m or less. Other examples include a pipette tip.

Device and apparatus for collecting microbial growth from a semi-solid surface

A system and method with increased sensitivity to microorganism growth. The system includes signal processing electronic circuit connected to a consumable or vessel through two or more electrodes that fully penetrate the vessel and are in contact with the fluid contents. The electronic circuit is configured to detect a component of the total impedance of the sample, specifically the 'out-of-phase' or imaginary reactance component, which has a sensitive response to organism growth in a frequency-dependent manner. The system detects changes in both the composition of charged molecules in the liquid matrix and the number of microorganisms based on monitoring the sample for change in this parameter. This results in a 5-70% reduction in time-to-detection (TTD). The system and method detect organisms in a plurality of vessel shapes, volumes, and matrix (or media) formats. The electrodes are fully immersed in a continuous body of liquid sample. The distance between electrodes may be adjusted or tuned to fit the needs of the vessel. The voltage inputs can also be adjusted to allow proper detection of the contents within the vessel.

An array of micro-chambers () with ISFETs () disposed therein for monitoring single cell activity in the microarray to determine the presence or absence of microorganisms in a sample ().

Methods and apparatus for rapid detection of infectious microorganisms

An array of micro-chambers () with individual ion sensitive field effect transistors (ISFETs) () disposed therein for monitoring single cell activity in the microarray to determine the presence or absence of microorganisms in a sample (). In addition to the presence or absence of a single cell, certain further embodiments contemplate monitoring cell behavior. Cell behavior includes the entire range of cell activity as well as cell response to changes in environmental conditions of changes in response due to the addition of sample constituents.

The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.

Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites

Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites

The present invention relates to compositions and methods for use in recombinational cloning of nucleic acid molecules. In particular, the invention relates to nucleic acid molecules encoding one or more recombination sites or portions thereof, to nucleic acid molecules comprising one or more of these recombination site nucleotide sequences and optionally comprising one or more additional physical or functional nucleotide sequences. The invention also relates to vectors comprising nucleic acid molecules of the invention, to host cells comprising vectors or nucleic acid molecules of the invention, to methods of producing polypeptides using nucleic acid molecules of the invention, and to polypeptides encoded by these nucleic acid molecules or produced by methods of the invention. The invention also relates to the use of these compositions in methods for recombinational cloning of nucleic acids, in vitro and in vivo, to provide chimeric DNA molecules that have particular characteristics and/or DNA segments.

Compositions for use in recombinational cloning of nucleic acids

The present invention relates generally to compositions and methods for use in recombinational cloning of nucleic acid molecules. In particular, the invention relates to nucleic acid molecules encoding one or more recombination sites or portions thereof, to nucleic acid molecules comprising one or more of these recombination site nucleotide sequences and optionally comprising one or more additional physical or functional nucleotide sequences. The invention also relates to vectors comprising the nucleic acid molecules of the invention, to host cells comprising the vectors or nucleic acid molecules of the invention, to methods of producing polypeptides using the nucleic acid molecules of the invention, and to polypeptides encoded by these nucleic acid molecules or produced by the methods of the invention. The invention also relates to antibodies that bind to one or more polypeptides of the invention or epitopes thereof. The invention also relates to the use of these compositions in methods for recombinational cloning of nucleic acids, in vitro and in vivo, to provide chimeric DNA molecules that have particular characteristics and/or DNA segments.

Compositions for use in recombinational cloning of nucelic acids

Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).

Recombinational cloning using nucleic acids having recombination sites

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Gaithersburg, MD, United States of America

Michael A Brasch