Expressive Research B.v.

Stichting Genetwister IP

Michaël Johannes Marcus Ebskamp

Anne Douwe De Boer

Ivo Laros

Simon Albertus Langeveld

Miranda Debora Van De Rhee

Joost Johannes Theodorus Gierkink

The invention relates to a method for analyzing a genomic region of an organism, comprising four major parts. The first part involves the isolation of mRNA from a selected organism that is used for the preparation of small single stranded DNA fragments with one adaptor containing an affinity label. These DNA fragments are used in part three. In the second part, genomic DNA from the same or a related organism is isolated. This genomic DNA is fragmented and ligated to adaptor molecules. In the third part, these genomic fragments are hybridized with single stranded DNA fragments from part one, and the hybrids formed in this process are used for synthesis of DNA fragments. These fragments will be used in part four which involves sequencing of these fragments using one of the available high throughput sequencing methods.

Expression-linked gene discovery

The present invention relates to a method for predicting the expected value of the quality features in agricultural and horticultural product and a method for predicting the expected optimal time of harvest by comparing expression parameters at the moment prior to harvest or during the postharvest path of genes and/or proteins related to such quality features for that agricultural and horticultural product, with (a) predetermined calibration line(s). The invention also comprises the markers M8, GAST and GDSL motif lipase and the uses thereof, as well as antibodies against them.

Determination of quality features in agricultural and horticultural crops

Growing community of inventors

Nieuwegein, Netherlands

Michaël Johannes Marcus Ebskamp