Ramot at Tel-aviv University Ltd.

Technion Research & Development Foundation Limited

Micha Fridman

Yuval Ebenstein

Ido M Herzog

Yoram Cohen

Roymon Joseph

Alissa Naugolny

Mark Feldman

Dana Kaizerman

Timor Baasov

Valery Belakhov

Sima Yaron

Cationic pillar[n]arenes, e.g., positively charged poly-ammonium, poly-phosphonium and poly-imidazolium pillar[5-6]arene derivatives are capable of inhibiting or preventing biofilm formation, and facilitating existing biofilm decomposition. A composition for inhibiting or disrupting biofilm, e.g., bacterial or fungal biofilm, formation, or reducing biofilm, can include a pharmaceutically acceptable carrier, and a cationic pillar[n]ene.

Method for inhibiting or disrupting biofilm formation, or reducing biofilm

Provided herein is a method of labeling the epigenetic modification-hydroxymethyl-cytosine along a DNA molecule that involves attaching a-hydroxymethyl-cytosine specific labeling agent to the DNA molecule, which is effected by enzymatically synthesizing UDP--N-Glucose by subjecting-azidoglucose to an enzymatic catalysis by N-acetylhexoseamine-kinase (NahK) in the presence of ATP to thereby obtain a phosphorylated-azidoglucose, followed by subjecting the phosphorylated-azidoglucose to enzymatic catalysis by uridyltransferase (GlmU) in the presence of UTP to thereby obtain UDP--N-Glucose, followed by incubating the DNA molecule with UDP--N-Glucose and ß-glucosyltransferase, to thereby obtain a-hydroxymethyl-cytosin e glycosylated by a-azidoglucose residue in the DNA molecule, and reacting the modified DNA molecule with a labeling agent which is chemically compatible with the azide group of the-azidoglucose residue.

Detection of hydroxymethylcytosine bases

The present invention provides cationic pillar[n]arenes, e.g., positively charged poly-ammonium, poly-phosphonium and poly-imidazolium pillar[5-6]arene derivatives, capable of inhibiting or preventing biofilm formation, and facilitating existing biofilm decomposition; as well as compositions thereof and methods of use.

Cationic pillararenes and uses thereof

Methodologies for labeling the epigenetic modification 5-hydroxymethylcytosine (5hmC) along a DNA molecule, and for determining a presence or a level of this epigenetic modification based on a ratio of fluorescence intensity of a labeled DNA sample to absorption intensity of the DNA sample at 260 nm are disclosed. Related compositions and reagents, and methods of preparing same are also disclosed.

Methodologies for labeling the epigenetic modification 5-hydroxymethyl-cytosine (5hmC) along a DNA molecule, and for imaging this epigenetic modification along a DNA molecule are disclosed. Related compositions and reagents, and methods of preparing same are also disclosed.

A novel group of aminoglycosides which share some structural features of currently available aminoglycosides with regard to the backbone, while also having significant structural differences, is disclosed. The similarity enables these aminoglycosides to be highly potent and effective antibiotics, while the significant differences enable these aminoglycosides to reduce or even block antibiotic resistance. The aminoglycosides of the present invention are suitable for inhibition of antrax lethal factor, hence are suitable for use as a cure for anthrax.

Growing community of inventors

Kiryat Bialik, Israel

Micha Fridman