Shenzhen Rhegen Biotechnology Co., Ltd.

Huawei Technologies Co., Limited

Miaomiao Zhang

Yong Hu

Guangjian Wang

Jia He

Rong Wen

Jingjing Cheng

Provided are a method for large-scale synthesis of a long-chain RNA and a method for site-specific modification of the long-chain RNA. The method for large-scale synthesis of a long-chain RNA comprises: designing short RNA fragments and splint DNA fragments; ligating; capping; and removing the splint DNA fragments and other steps. A large number of short RNA fragments and different splint DNA fragments are chemically synthesized, and then the different short RNA fragments are ligated by a biological method so as to form a target long-chain RNA. The product long-chain RNA has a low mutation rate, a plurality of the short RNA fragments can be assembled in a single reaction, and the long-chain RNA can be synthesized at a high throughput so as to fulfill the large-scale production of the long-chain RNA. In addition, by chemical modification of the short RNA fragments, the site-specific modification of the long-chain RNA can be realized.

Method for large-scale synthesis of long-chain RNA and method for site-specific modification of long-chain RNA

Disclosed herein are an mRNA targeting molecule comprising an N-acetylgalactosamine binding polypeptide and a preparation method therefor. A plasmid vector containing a DNA fragment formed by sequentially connecting a promoter, a target gene, a specific protease cleavage sequence, and a polynucleotide sequence encoding a GBD capable of binding to N-acetylgalactosamine, is transcribed to obtain an mRNA, which is connected to a DNA-puromycin linker under the action of a T4 ligase. The resulting connection product is subjected to protein translation, followed by cleavage using a specific protease to obtain an mRNA-puromycin-GBD complex, which then binds to a GBD protein sequence under the action of an N-acetylgalactosamine transferase to form an mRNA-puromycin-GBD-GalNAc complex, thereby modifying the mRNA with GalNAc, thus achieving the purpose of precise administration in a process of mRNA drug delivery and increasing the efficacy of the mRNA drug molecule.

mRNA targeting molecule comprising N-acetylgalactosamine binding polypeptide and preparation method therefor

The present invention provides a mannose-based mRNA targeted delivery system and use thereof. The mRNA can encode one or more target polypeptides and contains at least one mannose modification. The technical solution of the present invention modifies the mRNA molecule with a mannose, so that the mRNA can be directly and efficiently coupled with the mannose, and the targeted delivery of the mRNA is realized without the need for a carrier.

Mannose-based mRNA targeted delivery system and use thereof

Embodiments of the present application provide a beamforming based communications method and apparatus. The method includes: obtaining, by a transmit end, a transmit-end precoding matrix TrBB, a transmit-end intermediate-frequency beamforming matrix TrIF, and a transmit-end radio-frequency-end beamforming matrix TrRF according to feedback information that is from a receive end; acquiring a first data stream, and performing precoding processing on the first data stream according to TrBB, to generate a first analog signal; performing weighting and power amplification processing on the first analog signal according to TrIF, to generate a second analog signal; performing weighting and power amplification processing on the second analog signal according to TrRF, to generate a third analog signal; and determining an antenna array matching the third analog signal, and transmitting the third analog signal to the receive end by using the antenna array matching the third analog signal.

Beamforming based communications method and apparatus

The present invention provides a multi-channel array distortion compensation apparatus and method. The apparatus determines, according to a status of a first indication signal, whether to trigger a signal compensation operation; determines, according to a status of a second indication signal, a first adjustment condition is met, compares power of a received signal with a power threshold, and if the power of the received signal is greater than the threshold power, compensates the received signal according to a first adjustment factor; and if the status of the second indication signal does not meet the first adjustment condition, determines the status of the second indication signal meets a second adjustment condition, performs auxiliary compensation according to a second adjustment factor. By compensating signal distortion caused by chip stacking for a received signal, this reduces costs for implementing a multi-channel array system without the need to increase system complexity.

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Shenzhen, China

Miaomiao Zhang