Other

Wisconsin Alumni Research Foundation

Ophidian Pharmaceuticals, Inc.

Meyer B Jackson

Richard H Ffrench-Constant

Dongsheng Wang

Masoud Sepehri Rad

As described herein, a hybrid voltage sensor genetically-encoded voltage indicator (GEVI) for mitochondria or endoplasmic reticulum includes a transmembrane domain, and a fluorescent protein, wherein a terminus of the transmembrane domain and a terminus of the fluorescent protein are covalently linked directly or by a linker comprising 1 to 20 amino acids, and wherein the transmembrane domain comprises SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 or a peptide with greater than 85%, 90%, 95% or 98% identity to SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4. Also described are expression vectors, expression cassettes, and organelle membranes, as well as methods of determining the voltage across an organelle using the GEVIs.

Organelle-targeted genetically-encoded voltage indicators and methods of use thereof

An isolated polypeptide comprising a botulinum neurotoxin C1 light chain having a modified amino acid sequence relative to the sequence of a wild-type botulinum neurotoxin C1 light chain is disclosed. The modified botulinum neurotoxin C1 light chain is capable of selectively cleaving syntaxin protein, but has substantially reduced or no activity against SNAP-25 as compared to wild type botulinum neurotoxin C1 light chain, and is thus useful as a research tool and in medical treatment.

Engineered botulinum neurotoxin C1 with selective substrate specificity

The present invention relates to methods and reagents for drug and pesticide screening. Screening methods are disclosed employing normal and mutant receptors, allowing for the testing of large numbers of potentially useful compounds.

Drug and pesticide screening

Methods are described for regulating neuropeptide secretion to alleviate premature labor, hypertension, fluid imbalance, and risk of heart disease using neuroactive steroids targeted for a newly-identified site of action in the nerve terminals of neurosecretory neurons.

Method for regulating neuropeptide hormone secretion

Methods are described for regulating neuropeptide secretion to alleviate premature labor, hypertension, fluid imbalance, and risk of heart disease: using neuroactive steroids targeted for a newly-identified site of action in the nerve terminals of neurosecretory neurons. The steroids 17 betaestradiol and dehydroepiandrosterone increase the release of neuropeptide hormones such as oxytocin and vasopressin. Pregnalone derivatives decrease the release of the same hormones.

Growing community of inventors

Madison, WI, United States of America

Meyer B Jackson