Council of Scientific and Industrial Research

Meshram Harshdas Mitaram

Adari Bhaskar Rao

Alavala Sateesh

Madupathi Madhumala

Nandala Shiva Prasad

George Sara Anisa

Yvl Ravi Kumar

The invention relates to the development of a simple and eco-friendly process of extraction of sweet glycosides: steviosides from the leaves of. In particular, certain embodiments provide enhanced isolation of steviosides by enzyme assisted extraction, in combination with metal salts, followed by pressurized aqueous extraction. Thus obtainedextract was further processed for clarification and purification using multi-stage membrane filters (micro-filtration, ultra-filtration and nano membrane filtration) to obtain high purity steviosides from theleaves. This improved process confirms the isolation of high purity steviol glycosides i.e., Stevioside and Rebaudioside-A as the final products, with improved organoleptic properties. Thus, this superior process helps in obtaining pure steviosides from theleaf extract, without impurities and obnoxious residues, using easy operational technology without any damage to the environment.

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Telangana, India

Meshram Harshdas Mitaram