Cargill Inc.

Mervyn L deSouza

Paula M Hicks

James C Anderson

Sherry R Kollmann

Timothy Walter Abraham

Douglas Conrad Cameron, Jr

Steven John Gort

Sara C McFarlan

Fernando A Sanchez-Riera

John P Rosazza

Jose Miguel Laplaza

Mary Jo Zidwick

Jack Rosazza

Sara C Mcfarlan

Methods and compositions that can be used to make monatin or salt thereof from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.

Production of monatin and monatin precursors

Products and methods for the in vivo production of monatin sweetener are provided. The products include microorganisms that are genetically modified to secrete or to improve secretion of monatin; microorganisms that are genetically modified to produce monatin; and microorganisms that are genetically modified to both secrete or improve secretion of monatin and produce monatin. The methods include producing monatin in such genetically engineered microorganisms.

Products and methods for in vivo secretion of monatin

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Plymouth, MN, United States of America

Mervyn L deSouza