Valio Ltd.

Valio Oy

Valio, Finnish Co-Operative Dairies' Association

Mervi Sibakov

Ilkka Palva

Annika Mayra-Makinen

Soile Tynkkynen

Atte Von Wright

Teija Koivula

The invention relates to new Streptococcus thermophilus strains containing genes giving the bioluminescence property. The strains can be applied widely for analytical purposes, e.g. in the determination of bacteriophages and antibiotics, especially from liquid samples. The invention also relates to a test set containing a bioluminescent Streptococcus thermophilus strain, and to processes for the determination of bacteriophages or antibiotics from a liquid sample.

Streptococcus thermophilus strains and their use

DNA sequences, derived from Lactococcus lactis subsp. lactis, are useful as promoters and promoter/secretion promoting signals for heterologous or homologous expression in Gram-positive bacteria. In another aspect, the invention relates to vectors, such as plasmids, comprising the sequences of the present invention, and to host cells transformed with such vectors. Yet additional aspects of the present invention are related to methods for producing desired heterologous or homologous peptides or proteins employing the sequences, vectors, or transformed hosts of the invention. By means of the invention, greatly improved heterologous and homologous expression and secretion may be achieved in E. coli and in Gram-positive bacteria such as B. subtilis, Lactococcus and Lactobacillus.

Lactococcus promoters and signal sequences for heterologous gene

DNA sequences, derived from Lactococcus lactis subsp. lactis, are useful as promoters nd promoter/secretion promoting signals for heterologous or homologous expression in Gram-positive bacteria. In another aspect, the invention relates to vectors, such as plasmids, comprising the sequences of the present invention, and to host cells transformed with such vectors. Yet additional aspects of the present invention are related to methods for producing desired heterologous or homologous peptides or proteins employing the sequences, vectors, or transformed hosts of the invention. By means of the invention, greatly improved heterologous and homologous expression and secretion may be achieved in E. coli and in Gram-positive bacteria such as B. subtilis, Lactococci and Lactobacillus.

Lactococcus promoter and signal sequences for expression in bacteria

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Helsinki, Finland

Mervi Sibakov