The Upjohn Co.

Other

Merle G Wovcha

John C Knight

Candice B Biggs

Frederick J Antosz

Alfred B Garcia

Leo A Kominek

John M Beaton

Thomas R Pyke

Kevin E Brooks

Willard J Haak

A process for the microbiological conversion of steroids to (2+)-(7aS) 2,3,7,7-tetrahydro-7a-methyl-(1H)-indene-1,5(6H)-dione, hereinafter referred to as the indenedione.

Process for preparing an indenedione and a mycobacterium culture therefor

The subject invention concerns a novel fermentation process for making the useful steroid intermediate 24-nor-1,4-choladiene-3,22-dione (I). Compound (I) can be converted to valuable corticoids by known methods.

24-Nor-1,4-choladiene-3,22-dione

The subject invention concerns a novel one-stage fermentation process for making the useful steroid intermediate 9-hydroxy-3-oxo-4,17(20)-pregnadiene-20-carboxylic acid (I). This process is significantly superior to the best prior art process known for making (I).

Process of producing 9-hydroxy-3-oxo-4,17(20)-pregnadiene-20-carboxylic

The subject invention concerns a novel fermentation process for making the novel useful steroid intermediate 9-hydroxy-3-oxo-4,17(20)-pregnadiene-20-carboxyaldehyde (I). This compound can be used as in the synthesis of valuable corticoids.

Composition of matter and process

Mutant which is used in a novel microbiological process to selectively transform steroids having 17-alkyl side chains of from 2 to 10 carbon atoms, inclusive, to yield sole transformed product. AD is a valuable intermediate to make useful steroids.

Mycobacterium fortuitum mutant

Mutant which is used in a novel microbiological process to selectively degrade steroids having 17-alkyl side chains of from 2 to 10 carbon atoms, inclusive, to yield predominantly androst-4-ene-3,17-dione (AD) and small amounts of androsta-1,4-diene-3,17-dione (ADD). AD is a valuable intermediate to make useful steroids.

Microorganism mutant conversion of sterols to androsta-4-ene-3,17-dione

Mutant which is used in a novel microbiological process to selectively transform steroids having 17-alkyl side chains of from 2 to 10 carbon atoms, inclusive, to yield androst-4-ene-3,17-dione (AD) as essentially the sole transformed product. AD is a valuable intermediate to make useful steroids.

Mutants which are used in a novel microbiological process to selectively degrade steroids having 17-alkyl side chains of from 2 to 10 carbon atoms, inclusive, to androsta-1,4-diene-3,17-dione (ADD) and androst-4-ene-3,17-dione (AD). ADD and AD are valuable intermediates to make useful steroids.

Mycobacterium phlei mutants convert sterols to

Biologically pure culture of mutant mycobacterium

Novel compounds prepared by microbial transformation using novel mutants to selectively degrade steroids with or without 17-alkyl side chains of from 2 to 10 carbon atoms, inclusive. These compounds can be used as intermediates to make useful steroids.

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Kalamazoo, MI, United States of America

Merle G Wovcha