Valtion Teknillinen Tutkimuskeskus

Cargill Inc.

Teknologian Tutkimuskeskus Vtt Oy

Teknologian Tutkimuskeskus Vtt

Glykos Finland Oy

Natureworks LLC

Genencor International, Inc.

Vtt Technical Research Centre of Finland Ltd

Panimolaboratorio-bryggerilaboratorium

Danisco A/s

Stellenbosch University

Lallemand Hungary Liquidity Management LLC

Merja Penttilä

Laura Ruohonen

Kari Koivuranta

Vineet Rajgarhia

Peter Richard

Tiina Nakari-Setälä

Aristos Aristidou

Pirkko Suominen

Markku Saloheimo

Jari Natunen

Anne Kanerva

Juhani Saarinen

Ann Westerholm-Parvinen

Anne Huuskonen

Christopher K Miller

Stacey Olson

Mervi Toivari

Noora Salovuori

Anna-Liisa Hänninen

Markus Linder

Maria-Regina Kula

Hans Söderlund

John Londesborough

Michael C Ward

Liisa Viikari

Charles F Rice

Matti Siika-Aho

Jaakko Pere

Anne K Warner

Mark Mellon

Marja Ilmen

Christopher Landowski

Riaan Den Haan

Haowen Xu

Heikki Ojamo

Kristen M Deleault

Alan Benjamin Gilbert

Chris Miller

Indraneel Shikhare

Barbara A Swanson

Outi Koivistoinen

Harry Boer

Emile Van Zyl

Marja Ilmén

Jaana Uusitalo

Joosu Kuivanen

Maija Tenkanen

Dominik Mojzita

Danie Lagrange

Mira Povelainen

Richard Fagerström

Hanna Salo

Anita Teleman

Heli Viskari

Folke Tjerneld

Klaus Selber

Michael Bailey

Josef Deutscher

Deidre Willes

Jussi Joensuu

Auli Haikara

Tuija Kleemola

Elena Brevnova

Jim Flatt

Satu Hildich

Marilyn Wiebe

Satu Hilditch

Naomi Thorngren

Claire Plazanet-Menut

Andrei Miasnikov

Håkan Gros

Satu Kuorelahti

Jeff Villari

Alan Froehlich

Dan Murphy

Heidi Hanson Hau

Emily A Stonehouse

Jeffrey D Keating

Chhayal Ghandi

Shaunita Rose

The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.

Protease deficient filamentous fungal cells and methods of use thereof

The present invention relates to a method of converting oxalate to oxalyl-coA and/or oxalyl-coA to glyoxylate in a fungus and to a method of producing glycolic acid. Still, the present invention relates to a genetically modified fungus comprising increased enzyme activity associated with oxalyl-CoA. And furthermore, the present invention relates to use of the fungus of the present invention for producing oxalate, oxalyl-coA, glyoxylate and/or glycolic acid from a carbon substrate. Still furthermore, the present invention relates to a method of producing specific products and to a method of preparing the genetically modified fungus of the present invention.

Production of oxalyl-CoA, glyoxylate and/or glycolic acid

The present invention concerns a eukaryotic host selected from microorganisms, and a method for producing glycolic acid using said eukaryotic host cells, especially cells of a genetically modified fungal host. Further this invention relates to a glycolic acid product obtained using the method described here and the use of genetically modified microorganism cells in production of glycolic acid.

Eukaryotic cell and method for producing glycolic acid

The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to over-express genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein. A new enzyme having phospholipid: diacylglycerol acyltransferase (PDAT) activity and a polynucleotide encoding it are also disclosed, which are useful in the lipid production. A recombinantcell and its construction is described.

Genetically modified fungi and their use in lipid production

A DNA molecule comprising a fungal gene encoding an enzyme protein capable of converting L-galactonic acid into L-threo-3-deoxy-hexulosonic acid has been cloned and heterologously expressed. The enzyme is involved in the metabolic conversion of sugar acids, which are present in biological waste material such as sugar beet pulp and other pectin comprising material. A microorganism genetically modified to effectively express said enzyme may be used in fermenting biomaterial to desired end products such as ethanol. Alternatively, microorganisms in which the gene has been inactivated may be used to produce L-galactonic acid, which accumulates when the expression of the gene is prevented.

Microbial conversion of sugar acids and means therein

Provided is a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolyzed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is suitable for producing xylonic acid from a hemicellulose hydrolysate such as hydrolyzed lignocellulosic plant biomass.

Manufacture of xylonic acid

Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

Genetically modified yeast species, and fermentation processes using genetically modified yeast

Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications', include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

Genetically modified yeast species and fermentation processes using genetically modified yeast

A process for producing ethanol from a fibrous lignocellulosic raw material. After pre-treatment of the raw material, the fibrous fraction is first hydrolysed at high consistency and then the modified material is subjected simultaneously to continued hydrolysis with a cellulase and to ethanol fermentation in a fermentation mixture. Fermentation is continued to convert an essential portion of the available cellulose into ethanol, then a liquid fraction containing solubilized hemicelluloses is added to the fermentation mixture and fermentation continued. By means of the invention, high fermentation rates, high ethanol concentrations and low ethanol production costs can be attained.

Process for producing ethanol

A fungal microorganism can be engineered by means of genetic engineering to utilise L-arabinose. The genes of the L-arabinose pathway, which were unknown, i.e. L-arabinitol 4-dehydrogenase and L-xylulose reductase, were identified. These genes, together with the known genes of the L-arabinose pathway, form a functional pathway. This pathway can be introduced to a fungus, which is completely or partially lacking this pathway.

Engineering fungi for the utilisation of L-arabinose

This invention relates to a method for decreasing the foam formation during cultivation of a microorganism and to a method for producing an enhanced amount of a product of interest. The method comprises that the microorganism is modified in such a way that the microorganism does not produce an essential amount of at least one of the proteins, polypeptides or peptides associated with foam formation during cultivation of the unmodified microorganism. In particular the method comprises that the microorganism is modified not to produce an essential amount of amphipathic or hydrophobic proteins, polypeptides or peptides.

Method for decreasing the foam formation during cultivation of a microorganism

The present invention relates to isolation and purification of protein in aqueous two-phase systems (ATPS). Specifically, the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.

Process for partitioning of proteins

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Merja Penttilä