Upside Foods, Inc.

Meri Teresa Firpo

Nicholas J Genovese

Stephen K Hsu

Kalle Lukyan Johnson

Cameron Gentry Copeland

Kevin John Kayser

Patricia Rae Benton

Sukhdeep Singh Dhadwar

Robert Sierra

Marie Elizabeth Gibbons

Daphné Dambournet

Provided herein are nutrient media formulations and engineered growth factors, and methods thereof, useful for the production of slaughter-free meat.

Nutrient media for the production of slaughter-free meat

Provided herein are methods to increase the culture density and/or thickness of a cellular biomass in a cultivation infrastructure, to improve the culture of cells in the absence of serum in a cultivation infrastructure, and to promote anchorage-independent growth of a cellular biomass in a cultivation infrastructure. The methods comprise inhibiting the HIPPO signaling pathway, for example, by activating YAP1, activating TAZ, and/or inhibiting MOB1, LATS1 kinase, LATS2 kinase, WW45, MST1 kinase, and/or MST2 kinase in the cellular biomass. In some embodiments, the cellular biomass is harvested from the cultivation infrastructure for the formulation of cell-based food products or ingredients, such as animal meat manufactured from cells in an ex vivo process or for therapeutic applications such as organ or tissue transplantation or grafting.

Compositions and methods for increasing the culture density of a cellular biomass within a cultivation infrastructure

Growing community of inventors

Oakland, CA, United States of America

Meri Teresa Firpo