Bruker Daltonics, Inc.

Other

Bruker Daltonik Gmbh

Bruker Analytical Instruments, Inc.

The Texas A&M University System

Bruker Analytical Systems, Inc.

The University of North Carolina at Chapel Hill

Brucker Daltonics Inc.

Bruker Daltronics, Inc.

Florida State University Research Foundation, Inc.

Bruker Daltonics Gmbh & Co. Kg

Bruker Scientific LLC

Mann, Matthias

Melvin Andrew Park

Mark Ridgeway

Desmond Allen Kaplan

Oliver Räther

Houle Wang

Taeman Kim

Thomas Betz

Jochen Franzen

Michael Schubert

Frank H Laukien

Kevin Bernard Ray

Emile A Schweikert

Enio Frota Da Silveira

Yang Wang

Ulrich Geissmann

Craig M Whitehouse

Andreas Brekenfeld

Felician Muntean

Claus Koster

Matthias Mann

Gary Glish

Markus Lubeck

Christian Berg

Christian Bleiholder

John Wronka

Catherine Stacey

William D James

Fanny Caroline Liu

Victor George Fursey

Claus Koester

Fanny Liu

Desmond Kaplan

Kim Taeman

The invention provides a trapped ion mobility separator (TIMS) and methods to operate it wherein an ion region of the TIMS, through which ions travel along an axis from an entrance to an exit, has an elongate cross-sectional profile perpendicular to the axis with a long dimension and a short dimension. First and second counteracting forces on the ions along the axis are provided, wherein at least one of the first and second forces has an effect on the ions that is ion mobility dependent, and wherein at least one of the first and second forces varies spatially along the axis such that ions are trapped and separated by ion mobility. Different embodiments provide the first and second forces using different combinations of gas flow and electric field potential, and employ various electrode structures that provide the system with different advantageous characteristics.

Laterally-extended trapped ion mobility spectrometer

An ion mobility separator comprises an RF-device for transversely confining ions in an ion region using: (a) a first set of electrodes arranged parallel to one another along a direction of ion travel to define a first transverse boundary of the ion region, and that are supplied with a first RF-voltage such that different phases of the first RF-voltage are applied to adjacent electrodes of the first set; and (b) a second set of electrodes arranged parallel to one another along said direction of ion travel to define a second transverse boundary of the ion region, and that are supplied with a second RF-voltage such that different phases of the second RF-voltage are applied to adjacent electrodes of the second set, the first and second transverse boundaries being substantially opposite each other in a transverse direction of the ion region and the first and second RF voltages having different frequencies.

Dual-frequency RF ion confinement apparatus

The invention provides hybrid mass spectrometric systems which comprise an ion source, a first trapped ion mobility spectrometry (TIMS) analyzer and a mass analyzer, wherein the TIMS analyzer is located and operated in a first vacuum chamber at an elevated pressure above 500 Pa, and methods for operating the hybrid mass spectrometric systems.

Mass spectrometric system with ion mobility analyzer at elevated pressure

A mass spectrometric system comprises an RF-device for transversely confining ions in an ion region using: (a) a first set of electrodes arranged parallel to one another along a direction of ion travel to define a first transverse boundary of the ion region, and that are supplied with a first RF-voltage such that opposite phases of the first RF-voltage are applied to adjacent electrodes of the first set; and (b) a second set of electrodes arranged parallel to one another along said direction of ion travel to define a second transverse boundary of the ion region, and that are supplied with a second RF-voltage such that opposite phases of the second RF-voltage are applied to adjacent electrodes of the second set, the first and second transverse boundaries being opposite each other in a transverse direction of the ion region and the first and second RF voltages having different frequencies.

The invention relates to hybrid IMS/MS systems and provides hybrid IMS/MS system comprising an RF funnel, an ion mobility analyzer and a mass analyzer wherein the RF funnel is arranged non-collinearly to the ion mobility analyzer, preferably a TIMS analyzer (TIMS=trapped ion mobility spectrometry).

Hybrid mass spectrometric system

The invention is related to a trapped ion mobility spectrometer (TIMS device) and proposes to use higher order (order N&#x3e;2) linear multipole RF systems to accumulate and analyze ions at an electric DC field barrier, either pure higher order RF multipole systems or multipole RF systems with transitions from higher order towards lower order, e.g. from a linear octopolar RF system (N=4) to a linear quadrupole RF system (N=2) in front of the apex of the electric DC field barrier.

Trapped ion mobility spectrometer with high ion storage capacity

The invention proposes a methods and devices for tandem ion mobility spectrometry using at least one TIMS analyzers (TIMS=trapping ion mobility spectrometry), in particular in the field of structural biology.

Tandem ion mobility spectrometer

The invention proposes a mass spectrometer comprising two ion mobility analyzers in tandem arrangement, of which at least one is a trapped ion mobility spectrometer (TIMS), and an ion gate which is located between the two ion mobility analyzers, and use thereof wherein ions are selectively transferred between the two ion mobility analyzers by adjusting the transmission of the ion gate while ions are separated in time according to ion mobility in the first ion mobility analyzer.

Mass spectrometer with tandem ion mobility analyzers

The invention relates to the operation of ion mobility spectrometers based on gases pushing the ions over electrical field barriers, preferably in combination with mass spectrometers, and relates to trapped ion mobility spectrometers ('TIMS'). The invention proposes to accumulate and to scan the ions of a selected range of mobilities by using a long and flat electric field ramp created by additional voltages. By a voltage supplied at the beginning of the flat ramp, the lowest mobility of the mobility range of ions to be collected can be selected. By the difference of the voltages at the beginning and the end, the width of the mobility range is determined. The spatial zoom advantageously can collect considerable more ions of interest than a temporal zoom without severe losses by space charge effects, and more ions can be detected in the mass-mobility map.

Spatial zoom mode for accumulative trapped ion mobility spectrometry

The invention relates to the linear dynamic range of ion abundance measurement devices in mass spectrometers, such as time-of-flight mass spectrometers. The invention solves the problem of ion current peak saturation by producing a second ion measurement signal at an intermediate stage of amplification in a secondary electron multiplier, e.g. a signal generated between the two multichannel plates in chevron arrangement. Because saturation effects are observed only in later stages of amplification, the signal from the intermediate stage of amplification will remain linear even at high ion intensities and will remain outside saturation. In the case of a discrete dynode detector this could encompass, for example, placement of a detection grid between two dynodes near the middle of the amplification chain. The invention uses detection of the image current generated by the passing electrons.

High dynamic range ion detector for mass spectrometers

The invention provides a method for acquiring fragment ion spectra of substances in complex substance mixtures wherein a trapped ion mobility spectrometer ('TIMS') is used as the ion mobility separator separation device. The fragment ion spectra may be used for the identification of high numbers of proteins in complex mixtures, or for a safe quantification of some substances, by their fragment ion mass spectra in a mass spectrometer with up-front substance separator. TIMS with parallel accumulation provides the unique possibility to prolong the ion accumulation duration to find more detectable ion species without decreasing the measuring capacity for fragment ion mass spectra. The high measurement capacity for fragment ion mass spectra permits the repeated measurement of low abundance ion species to improve the quality of the fragment ion spectra.

Acquisition of fragment ion mass spectra of ions separated by their mobility

The invention provides a method for acquiring fragment ion spectra of substances in complex substance mixtures wherein a trapped ion mobility spectrometer ('TIMS') is used as the ion mobility separation device coupled to a triple quadrupole mass filter assembly. The fragment ion spectra may be used for the identification of high numbers of proteins in complex mixtures, or for a safe quantification of some substances, by their fragment ion mass spectra in a mass spectrometer with upstream substance separator. TIMS, in particular equipped with parallel accumulation, provides the unique possibility to prolong the ion accumulation duration to find more detectable ion species without decreasing the measuring capacity for fragment ion mass spectra. The high measurement capacity for fragment ion mass spectra permits the repeated measurement of low abundance ion species such as to improve the quality of the fragment ion spectra.

Triple quadrupole mass spectrometry coupled to trapped ion mobility separation

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Billerica, MA, United States of America

Melvin Andrew Park