Becton Dickinson and Company Limited

Melody M H Kuroda

Michael L Marshall

Michael Justin Lizzi

M Scott Ulrich

Dwight Livingston

Thomas D Haubert

James A Prescott

Adam C Curry

Gregory S Kramer

Randy J Somerville

Ammon David Lentz

Scott Nicholas Danhof

Artis R Lockhart

Jeffrey S Krause

Ernie Ritchey

Kristin Weidemaier

Eric Fallows

Erin Gooch Carruthers

Mark A Talmer

Robert L Campbell

Jack H Kessler

Andrea Liebmann-Vinson

Kevin G Dolan

David S Sebba

Donald E Gorelick

Miroslaw Bartkowiak

Spencer Lovette

Wendy Dale Woodley

Jeffrey S Ojala

Scott N Danhof

Jack Kessler

Vessel assembly

Provided herein are methods, systems, and devices for detecting and/or identifying one or more specific microorganisms in a culture sample. Indicator particles, such as surface enhanced Raman spectroscopy (SERS)-active nanoparticles, each having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest, can form a complex with specific microorganisms in the culture sample. Further, agitating magnetic capture particles also having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest can be used to capture the microorganism-indicator particle complex and concentrate the complex in a localized area of an assay vessel for subsequent detection and identification. The complex can be dispersed, pelleted, and redispersed so that the culture sample can be retested a number of times during incubation so as to allow for real-time monitoring of the culture sample.

Methods, systems, and devices for detecting and identifying microorganisms in microbiological culture samples

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Vessel

Vial assembly

Cap portion of a vial

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Durham, NC, United States of America

Melody M H Kuroda