Emd Millipore Corporation

Merck Patent Gmbh

Melissa Holstein

Nanying Bian

Ronald Tuccelli

Christopher Gillespie

John Caulmare

Matthew T Stone

Santosh Rahane

Luc Messier

Kristen Cotoni

Roger Hill

Chia-Yun Sun

Embodiments described herein relate to novel chromatography media for removing anti-A and/or anti-B antibodies from a sample, as well as methods of using the same. The media described herein have several advantages over previously described media including, acid and alkaline stability.

Affinity chromatography media for removal of anti-a and/or anti-b antibodies

Methods of maintaining narrow residence time distributions in continuous flow systems, particularly applicable to virus inactivation such as during a protein purification process. Fluid sample is introduced into an axial flow channel and caused to flow therein in discrete packets or zones to minimize residence time distribution and axial dispersion. Embodiments described herein obviate or minimize the need for using large tanks or reservoirs for performing virus inactivation during a protein purification process; reduce the overall time required for virus inactivation, and/or reduce the overall physical space required to perform the virus inactivation operation during a protein purification process, which in turn reduces the overall footprint for the purification process.

Mechanical method of maintaining narrow residence time distributions in continuous flow systems

Method of maintaining narrow residence time distributions in continuous flow systems

The present invention provides methods for increasing purity of an Fc-containing protein by removing protein aggregates during the Protein A chromatography step used during the purification of the Fc-containing protein.

Methods of increasing protein purity using protein A based chromatography

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Concord, MA, United States of America

Melissa Holstein