E.i. Dupont De Nemours and Company

Melissa D Bosak

Pamela L Sharpe

Quinn Qun Zhu

Qiong Cheng

Narendra S Yadav

Zhixiong Xue

Luan Tao

Hongxian He

Hongxiang Zhang

Seung-Pyo Hong

Dana M Walters Pollak

Natalia Sedkova

Michael W Bostick

Yehong Jamie Wang

Mutant delta-5 desaturases, having the ability to convert dihomo-gamma-linolenic acid [DGLA; 20:3 omega-6] to arachidonic acid [ARA; 20:4 omega-6] and/or eicosatetraenoic acid [ETA; 20:4 omega-3] to eicosapentaenoic acid [EPA; 20:5 omega-3] and possessing at least one mutation within the HPGG (SEQ ID NO:7) motif of the cytochome b-like domain and at least one mutation within the HDASH (SEQ ID NO:8) motif are disclosed. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturases, along with a method of making long chain polyunsaturated fatty acids ['PUFAs'], are also disclosed.

Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids

Methanotrophic bacterial strains are provided that have been optimized for the production of carotenoid compounds through the down-regulation of one or more of the crtN1, ald, crtN2 and crtN3 genes of the carotenoid biosynthetic pathway. The resulting strains lack pigmented Ccarotenoid compounds, and show an increase in the production of Ccarotenoids. The use of the optimized host strains for the production of the Ccarotenoids canthaxanthin and astaxanthin is also described.

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Growing community of inventors

Mount Royal, NJ, United States of America

Melissa D Bosak