Life Technologies Corporation

Melissa Barker

Pius Brzoska

Manohar R Furtado

Craig Cummings

Olga Petrauskene

Paolo Vatta

Lily Wong

Craig A Cummings

Disclosed are methods and kits for the specific detection ofO157:H7 and notO55:H7 from samples such as: complex food matrices, water, beverages, fermentation broths, forensic & biological samples, and environmental samples including food processing and manufacturing surfaces. In some embodiments, a method of the disclosure comprises: hybridizing at least a first pair of polynucleotide primers to at least a first target polynucleotide sequence, hybridizing at least a second pair of polynucleotide primers to at least a second target polynucleotide sequence, amplifying the at least first and at least second target polynucleotide sequences, and detecting the first and second amplified target polynucleotide sequence products, wherein the detection of both the first amplified target polynucleotide sequence product and the second amplified target polynucleotide sequence product is indicative of the presence ofO157:H7 in a sample and notO55:H7.

[object Object]

Disclosed are primer and probe compositions, PCR assays, methods and kits for the specific detection ofO157:H7 and notO55:H7 from a variety of samples. In some embodiments, methods for specifically detectingO157:H7 comprise: hybridizing at least a first pair of polynucleotide primers to at least a first target polynucleotide sequence, hybridizing at least a second pair of polynucleotide primers to at least a second target polynucleotide sequence, amplifying said at least first and said at least second target polynucleotide sequences, and detecting said at least first and said at least second amplified target polynucleotide sequence products, wherein the detection of both the first amplified target polynucleotide sequence product and the second amplified target polynucleotide sequence product is indicative of the presence ofO157:H7 in a sample and notO55:H7.

Growing community of inventors

Gilroy, CA, United States of America

Melissa Barker