Georgia Tech Research Corporation

Basf Se Corporation

Texaco Inc.

Evonik Degussa Gmbh

Melanie Hall

Kurt Faber

Prabuddha Bansal

Jay Hyung Lee

Thomas Haas

Bernhard Hauer

Rainer Stürmer

Thomas Friedrich

Andreas Sebastian Bommarius

Anja Thiessenhusen

Matthew James Realff

Thomas Buelter

Alexander Dennig

Ronald W Smith

Stefan Gilch

Andreas Sebatian Bommarius

The present invention provides a microbial cell capable of producing at least one terminal alkene from at least one short chain fatty acid, wherein the cell is genetically modified to comprise

Alkene production

In one embodiment the instant invention generally pertains to a method for producing glucose for fermentation. The method comprises first treating a biomass with acid and heat under conditions sufficient to produce a composition mixture comprising cellulose suitable for enzymatic hydrolysis. Next, at least a portion of the cellulose of step (a) is enzymatically hydrolyzed under conditions sufficient to form a composition comprising glucose. The glucose is then fermented. Advantageously, one or more reaction conditions are more efficient because they are selected by first measuring an initial hydrolysis rate of said biomass and then selecting one or more appropriate reaction conditions based upon said initial hydrolysis rate.

Methods of enzymatic hydrolysis

The present invention is a process for treating a feedstock comprising holocellulose. The process comprises mixing the feedstock with a solution comprising cellulose binding domains to form a mixture. The mixture is then subjected to conditions sufficient to reduce the crystallinity of holocellulose. Subsequent enzymatic hydrolysis may show an improved rate and/or fermentable sugar yield as compared to processes which do not employ the process.

Methods of treating a biomass for enzymatic hydrolysis

A method for enzymatic preparation of compounds of the general formula (2) from unsaturated alkene derivatives of the general formula (1) by reducing a compound of the formula (1) in the presence of a reductase, comprising at least one of the polypeptide sequences SEQ ID NO: 1, 2 or 3 or having a functionally equivalent polypeptide sequence which is at least 80% identical to SEQ ID NO: 1, 2 or 3.

Process for enzymatic reduction of alkene derivatives

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Graz, Austria

Melanie Hall