Illumina Cambridge Limited

Illumina Corporation

Melanie Anne Smith

Niall Anthony Gormley

David James Earnshaw

Helen Rachel Bignell

The invention relates to a method of preparing a library of template polynucleotides with uniform sequence representation and to use of a library of templates prepared using this method for solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a library of template polynucleotides which have common sequences at their 5' ends and at their 3&#x2032; ends, which contains even representation of all the fragments present in a starting sample of nucleic acid before fragmentation. The invention is especially applicable to the preparation of short insert libraries, where the sample fragments are less than 150 base pairs in length.

Method for retaining even coverage of short insert libraries

The invention relates to a method of preparing a library of template polynucleotides which reduces and/or prevents the formation of adaptor-dimers. The invention also relates to the use of a library of templates prepared using the method of the invention for solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a library of template polynucleotides which have common sequences at their 5' ends and at their 3&#x2032; ends which is substantially free of adaptor-dimers.

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Nr Saffron, United Kingdom

Melanie Anne Smith