Jiangnan University

Meiqi Wu

Xian Zhang

Zhiming Rao

Meijuan Xu

Taowei Yang

Minglong Shao

Jianing Zhang

Yaling Wang

Jiamin Chen

Anqi Peng

Shuping Xu

The present invention provides an aspartase mutant, a recombinant expression vector and recombinant bacterium containing the aspartase mutant, and the use thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the aspartase mutant is as set forth in SEQ ID NO: 1. In the aspartase mutant of the present invention, on the basis of wild type aspartase (with an amino acid sequence as set out in SEQ ID NO: 3), glutamic acid at position 427 is mutated into glutamine. In the present invention, by mutating the amino acid residue at position 427 into glutamine, the polar environment near an active site is changed, and thus ammonia supply during substrate reaction is further facilitated, thereby improving an enzyme activity, enhancing the ability of the enzyme in synthesizing a &#x3b2;-amino acid, and providing a practical and effective strategy for industrial production of the &#x3b2;-amino acid.

Aspartase mutant, recombinant expression vector and recombinant bacterium containing aspartase mutant, and use thereof

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Jiangsu, China

Meiqi Wu