Beth Israel Deaconess Medical Center, Inc.

Meiheng Yang

Arthur J Sytkowski

The invention provides isolated nucleic acids molecules, designated NPG-1 nucleic acid molecules, which encode polypeptides involved in the modulation of a response in a tumor cell. The invention also provides antisense nucleic acid molecules, expression vectors containing NPG-1 nucleic acid molecules, host cells into which the expression vectors have been introduced, and non-human transgenic animals in which an NPG-1 gene has been introduced or disrupted. The invention still further provides isolated NPG-1 polypeptides, fusion polypeptides, antigenic peptides, and anti-NPG-1 antibodies. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.

NPG-1 gene that is differentially expressed in prostate tumors

Methods for identifying differentially expresses genes and differences between genomic nucleic acid sequences are described. These methods typically include: (a) providing a tester DNA molecule with an amplification tag at the 5' and 3' ends of the molecule and a driver DNA or RNA molecule lacking said amplification tag; (b) hybridizing said tester and said driver molecules to form a reaction mixture, wherein said reaction mixture comprises a tester--tester homoduplex, a tester-driver heteroduplex, a driver--driver homoduplex, a single stranded driver DNA molecule and a single stranded tester DNA molecule; (c) treating said reaction mixture to reduce the number of single stranded molecules in said mixture; (d) treating said reaction mixture to remove said amplification tag from said tester-driver heteroduplex; and (e) amplifying said tester--tester homoduplex from said reaction mixture to form an amplification product, wherein steps (c) and (d) occur before step (e).

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Norwood, MA, United States of America

Meiheng Yang