Nantong University

Chia Tai Tianqing Pharmaceutical Group Co., Ltd.

Centaurus Biopharma Co., Ltd.

Lianyungang Runzhong Pharmaceutical Co., Ltd.

Mei Liu

Bo Liu

Yong Peng

Yongxin Han

Hong Luo

Yuandong Hu

Xiaosong Gu

Xijie Liu

Fei Ding

Shulong Wang

Yumin Yang

Yan Liu

Xiaodong Wang

Kuncheng Chen

Tuchen Guan

Peiyun Zhang

Xuemei Chen

A silk fibroin nerve graft fused with NT3 and a preparation method therefor is provided. The method includes the steps of: synthesizing a gene fragment containing a silk fibroin light chain and NT-3, connecting the fragment to a pET-30 expression vector, and transferring the obtained recombinant expression vector into BL21to obtain a fusion protein; placing a silk fibroin fiber web in a mold, mixing the fusion protein and a silk fibroin solution, and performing freeze drying to enable the silk fibroin to be crosslinked with the fusion protein to form a nerve conduit; and after deformation processing, finally obtaining a silk fibroin nerve graft having NT-3 activity. The silk fibroin nerve graft can provide a mechanical support, exert nerve protection and nerve regeneration promotion functions in a long term, and adjust the proportion of NT-3 bioactive peptides in the nerve conduit, facilitating repair of nerve injuries.

Preparation method for silk fibroin nerve graft fused with NT3

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Jiangsu, China

Mei Liu