The General Hospital Corporation

Gpb Scientific, LLC

Verinata Health, Inc.

Massachusetts Institute of Technology

Other

University of Illinois

Organogenesis, Inc.

Daktari Diagnostics, Inc.

Purdue Research Foundation

University of Massachusetts

The General Hospital Coporation

Gpr Scientific, LLC

Mehmet Toner

Ravi Kapur

Kyle C Smith

Daniel Irimia

Jon F Edd

Thomas Alan Barber

Roland B Stoughton

Shannon Stott

Daniel D Shoemaker

Dino DiCarlo

Ronald W Davis

Zihua Wang

Martin L Yarmush

Bruce L Carvalho

Lotien Richard Huang

George Truskey

Paul J Vernucci

Fabio Fachin

Ramin Haghgooie

Barb Ariel Cohen

Xuanhong Cheng

Shannon Tessier

Eduardo Reategui

William Rodriguez

Robert Marius Bieganski

Thomas L Toth

Rashid Bashir

Martin Fuchs

Mustafa Korkut Uygun

Ajay Shah

Lindong Weng

Vincent Pai

Grace Chen

Philipp S Spuhler

Ismail Emre Ozkumur

Sangeeta N Bhatia

Brian Lee Wardle

Utkan Demirci

Chia-Hsien S Hsu

Maria-Louisa Izamis

Aaron Oppenheimer

Basak Elif Uygun

Stephen R Watson

Nicholas Watkins

Alex J Fowler

Arno W Tilles

Eugene J Lim

Ulysses J Balis

Michael Russo

Ali Eroglu

Nezihi Murat Karabacak

Rebecca Sandlin

Keith Wong

John Walsh

Timothy Antonie Berendsen

Osman Berk Usta

Sinem Perk

Yi-Shao Liu

Martin Arnold Schmidt

Robert E Cohen

Daniel Arie Haber

Lee Zamir

Gareth H McKinley

Michael F Rubner

Kristi S Anseth

Patrick Seamus Doyle

Shyamala Maheswaran

Maury D Cosman

Joel Voldman

Daniel Colin Pregibon

Paul A DiMilla

David Tomoaki Miyamoto

Alexander G Tschumakow

Martha L Gray

Xin Hong

Tanya Todorova

Thomas J Ober

Mark W Tibbitt

April M Kloxin

Baris R Mutlu

Stephanie E J Cronin

Casie A Pendexter

Xiaocheng Jiang

Gloria Y Lee

Thomas A Barber

Sarah Javaid

Kaja Kaastrup

Reinier Johan De Vries

Murat N Karabacak

Xiaoming He

John Oakey

Rebecca Braff

Liju Yang

Roland Stoughton

The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.

Devices and method for enrichment and alteration of cells and other particles

Microfluidic systems are configured to process liquid samples that include cumulus-oocyte complexes (COCs), such as raw follicular fluid, in an automated and continuous manner to produce oocytes separate or 'denuded' from surrounding cumulus cells. The systems include a substrate and a channel that can have two or more sub-channels. Each channel includes an inlet, an outlet, and one or more stages arranged in series. Each of the stages includes one or more expansion units and one or more constriction units. At least one stage includes constriction units having jagged internal surfaces, e.g., teeth, that help remove the cumulus cells from the COCs.

Microfluidic systems and methods to denude mammalian oocytes

Methods of using particle size amplification to facilitate size-based particle separation and concentration. At least one of the methods includes introducing a plurality of binding moieties into a fluid sample; allowing at least one of the binding moieties to bind two or more biological particles to form a particle cluster, in which the particle cluster includes a first type of biological particle bound to a second different type of biological particle; and flowing the fluid sample including the particle cluster into a particle sorting region of a microfluidic device.

Size-based particle separation and concentration using particle size amplification

The present invention generally relates to methods and compositions to determine viability of an organ for transplantation and other medical purposes. One aspect of the invention relates to a method for assessing the viability of an organ by measuring the energy parameters to determine the energy level of the organ by determining the stored cellular energy (e.g., ATP levels), and/or energy consumption over a particular time period of viability. The energy parameters can be compared to reference energy parameters as a highly accurate and reliable prediction of viable cell yield, and organ viability. Another aspect of the invention relates methods to preserve or extend the time period of viability of an organ any combination of (i) preservation perfusion of the organ to prevent ischemic damage, (ii) chemical metabolic suppression of the organ e.g., using metabolic suppressants, (iii) metabolic suppression by physical or environmental conditions, e.g., sub-zero non-freezing storage.

Methods and compositions for preserving tissues and organs

A microfluidic device includes: a first microfluidic channel; a second microfluidic channel extending along the first microfluidic channel; and a first array of islands separating the first microfluidic channel from the second microfluidic channel, in which each island is separated from an adjacent island in the array by an opening that fluidly couples the first microfluidic channel to the second microfluidic channel, in which the first microfluidic channel, the second microfluidic channel, and the islands are arranged so that a fluidic resistance of the first microfluidic channel increases relative to a fluidic resistance of the second microfluidic channel along a longitudinal direction of the first microfluidic channel such that, during use of the microfluidic device, a portion of a fluid sample flowing through the first microfluidic channel passes through one or more of the openings between adjacent islands into the second microfluidic channel.

Concentrating particles in a microfluidic device

This disclosure is related to methods of preserving biological samples, organs, and organisms. This disclosure is based, at least in part, on the discovery that in the presence of heterogenous extracellular ice, endothelial cells have improved attachment and viability after preservation, and particularly by uniformly nucleating ice across the microvasculature using ice nucleating agents, the chance of intracelluar ice formation can be significantly reduced.

High subzero cryopreservation

Systems, methods, and devices for selective capture and release of target particles, e.g., living cells, from liquid samples, e.g., blood, are provided. The particle capture systems include a substrate; a first layer of gelatin bound to the substrate by physical adsorption, wherein the gelatin is functionalized with a plurality of first members of a binding pair; a second layer of gelatin wherein the gelatin is functionalized with a plurality of the first members of the binding pair and the second layer is bound to the first layer via a plurality of second members of the binding pair that are associated with the first members of the binding pair on both the first and the second layers; and a plurality of nanostructures bound to the second members of the binding pair and to one or more particle-binding moieties that selectively bind to the target particles.

Capture and release of particles from liquid samples

A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.

Sorting particles in a microfluidic device

This disclosure relates to new assay methods for analysis of circulating tumor cells (CTCs) in blood samples for detection, e.g., early detection, and/or monitoring of disease, e.g., cancer. The methods provide ultra-high sensitivity and specificity, and include the use of microfluidic isolation of CTCs and digital detection of RNA derived from the CTCs.

Digital analysis of circulating tumor cells in blood samples

The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.

Rare cell analysis using sample splitting and DNA tags

Microfluidic devices are described that include a microfluidic channel, a first array of one or more magnets above the microfluidic channel, each magnet in the first array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the first array, and a second array of one or more magnets beneath the microfluidic channel, each magnet in the second array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the second array. The first array is aligned with respect to the second array such that magnetic fields emitted by the first array and second array generate a magnetic flux gradient profile extending through the channel. An absolute value of the profile includes a first maximum and a second maximum that bound a local minimum. The local minimum is located within the microfluidic channel or less than 5 mm away from a wall of the microfluidic channel. Methods of using the new devices are also described.

Microfluidic sorting using high gradient magnetic fields

The present invention provides apparatus and methods for enriching components or cells from a sample and conducting genetic analysis, such as SNP genotyping to provide diagnostic results for fetal disorders or conditions.

Fetal aneuploidy detection by sequencing

Methods for stabilizing blood samples, e.g., clinical blood samples, for storage or transportation before use.

Stabilization of whole blood samples

Systems and techniques are described for capturing target extracellular vesicles from a fluid sample. In some implementations, a microfluidic device includes a microfluidic channel where an internal surface of at least one wall of the microfluidic channel includes a plurality of grooves or ridges, or both grooves and ridges, arranged and configured to generate chaotic mixing within a fluid sample flowing through the microfluidic channel. The microfluidic device also includes a plurality of elongate flexible linker molecules, each having a molecular weight between about 1.8-4.8 kDa, where each elongate flexible linker molecule is bound at a first end to an internal surface of at least one wall of the microfluidic channel and is bound at a second end to one or more binding moieties that specifically bind to a target extracellular vesicle.

Engineered nano-interfaces for microfluidic isolation of extracellular vesicles

Growing community of inventors

Charlestown, MA, United States of America

Mehmet Toner