Ajinomoto Co., Ltd.

Megumi Shimaoka

Hisashi Kawasaki

Osamu Kurahashi

Hiroshi Matsui

Yasuhiro Takenaka

Yoshihiro Usuda

Hisashi Yasueda

Shinichi Sugimoto

Masakazu Nakazawa

Yasuhisa Asano

Norimasa Onishi

Yasuhiro Mihara

Nobuharu Tsujimoto

Yoshiya Gunji

Ikuo Kira

Takashi Utagawa

Kohki Ishikawa

Ei-ichiro Suzuki

Nobuhisa Shimba

Keiko Gondoh

Tohru Kouda

Yuri Miyata

Manami Oba

Rie Kozutsumi

A microorganism which has a gene encoding an enzyme in which feedback inhibition is desensitized by substitution of one or two amino acids in PRPP amidotransferase encoded by purF of, a gene encoding a protein which is an inactivated repressor of purine nucleotide biosynthesis encoded by purR, a gene encoding an enzyme which is inactivated purine nucleoside phosphorylase encoded by deoD, a gene encoding an enzyme which is inactivated succinyl-AMP synthase encoded by purA, a gene encoding an enzyme which is inactivated 6-phosphogluconate dehydrase encoded by edd, a gene encoding an enzyme which is inactivated phosphoglucose isomerase encoded by pgi and like is bred and a purine nucleoside is produced by culturing the microorganism.

Method for producing purine nucleoside by fermentation

An L-amino acid is produced by culturing abacterium which can grow by using methanol as a main carbon source and has L-amino acid-producing ability, for example, abacterium in which dihydrodipicolinate synthase activity and aspartokinase activity are enhanced by transformation through introduction into cells, of a DNA coding for dihydrodipicolinate synthase that does not suffer feedback inhibition by L-lysine and a DNA coding for aspartokinase that does not suffer feedback inhibition by L-lysine, or abacterium made to be casamino acid auxotrophic, in a medium containing methanol as a main carbon source, to produce and accumulate an L-amino acid in culture, and collecting the L-amino acid from the culture.

[object Object]

There is provided a mutant nucleoside-5'-phosphate producing enzyme with improved nucleoside-5&#x2032;-phosphate producing ability of a nucleoside-5&#x2032;-phosphate producing enzyme that has transphosphorylation activity and/or phosphatase activity and has one Lys residue, two Arg residues and two His residues with distances between their C&#x3b1;'s within a particular ranges and a space around them allowing a binding of a nucleoside, of which mutation is designed by using the crystal structure of EB-AP.

Variant nucleoside-5'-phosphate producing enzyme

The present invention relates to a process for producing 5&prime;-inosinic acid or 5&prime;-guanylic acid for use in seasonings or the like from inosine or guanosine or a precursor thereof using an adenosine triphosphate (ATP)-regenerating microorganism containing a DNA encoding a protein that has the activity of forming 5&prime;-inosinic acid or 5&prime;-guanylic acid from inosine or guanosine. Further, the present invention relates to a novel protein having the inosine-guanosine kinase activity, a gene encoding said protein, a recombinant DNA containing said gene, and a microorganism which is transformed with said recombinant DNA.

Process for producing nucleic acids

The present invention provides a method of the production of (S)-.gamma.-halogenated-.beta.-hydroxybutyric acid esters at a high accumulation and a high yield. A microorganism belonging to the genus Stemphylium, Alternaria, Corynespora or Torulaspora is used to asymmetrically reduce a .gamma.-halogenated-acetoacetic acid ester into an (S)-.gamma.-halogenated-.beta.-hydroxybutyric acid ester, and the (S)-.gamma.-halogenated-.beta.-hydroxybutyric acid ester produced is recovered from the reaction mixture.

Method of the production of (s)-gamma-halogenated-.gamma.-hydroxybutyric

Growing community of inventors

Kawasaki, Japan

Megumi Shimaoka