Degussa Aktiengesellschaft

Evonik Degussa Gmbh

Other

Evonik Operations Gmbh

Degussa Gmbh

Mechthild Rieping

Thomas Hermann

Walter Pfefferle

Georg Thierbach

Lothar Eggeling

Stephan Hans

Miroslav Patek

Bettina Mockel

Mike Farwick

Hermann Sahm

Achim Marx

Caroline Kreutzer

Nicole Dusch

Christine Bastuck

Nicole Siebelt

Sahm Hermann

Caroline Kruetzer

Brigitte Bathe

Alfred Puhler

Bettina Möckel

Douwe Molenaar

Michel Eduard Van Der Rest

Birgit Witteck

Indra Schr&ouml;der

Caroline Gerth

The invention relates to a recombinant, L-amino acid-secreting microorganism of the Enterobacteriaceae family, comprising an DNA fragment having promoter activity that is functionally linked to a polynucleotide coding for a membrane protein, characterized in that the DNA fragment having promoter activity comprises the SEQ ID NO: 8.

Process for preparing L amino acids using improved strains of the enterobacteriaceae family

Alleles of the thrA gene from Enterobacteriaceae encoding desensitized aspartokinase I-homoserine dehydrogenase I enzymes and methods for the fermentative production of L-threonine using bacteria containing these alleles.

Alleles of the thrA gene of Enterobacteriaceae

The invention relates to a process for the preparation of L-amino acids, in particular L-threonine.

Process for L-amino acid production using enterobacteriaceae with over-expression of ptsG gene

L-lysine-producing strains ofwith enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and a process for the preparation of L-lysine.

L-lysine-producing corynebacteria and process for the preparation of L-lysine

The invention relates to a method for the production of L-amino acids by fermentation of recombinant microorganisms of the Enterobacteriaceae family, characterized in that a) the microorganisms producing the desired L-amino acid and wherein the lamB-gene or nucleotide sequence coding for the gene product maltoporin is amplified, particularly overexpressed, are cultivated in a medium in conditions enabling the desired L-amino acid to be enriched in the medium or in cells, and b) the desired L-amino acid is isolated, wherein constituents of the fermentation broth and/or biomass remain in the entirety thereof or in parts thereof (=0 bis 100%) in the isolated product or are fully removed.

Method for the production of L-amino acids using strains from the Enterobacteriaceae family which contain an enhanced lamb gene

The present invention relates to a fermentation process for the preparation of L-amino acids in which the following steps are carried out: (a) fermentation of the microorganisms of the family Enterobacteriaceae producing the desired L-amino acid, in which microorganisms' open reading frames of yjfA and ytfP are individually or jointly inactivated by one or more methods of mutagenesis selected from the group consisting of deletion, insertional mutagenesis due to homologous recombination, and transition or tranversion mutagenesis with incorporation of a non-sense mutation in the yjfA and ytfP gene region; (b) concentration of the fermentation broth to eliminate water and increase the concentration of said L-amino acids; and (c) isolation of the L-amino acids.

[object Object]

The invention relates to a process for the preparation of L-amino acids, in particular L-threonine, in which the following steps are carried out: a) fermentation of microorganisms of the Enterobacteriaceae family which produce the desired L-amino acid and in which the yjgF ORF or the nucleotide sequence which codes for it are attenuated, in particular eliminated, b) concentration of the L-amino acid in the medium or in the cells of the bacteria, and optionally c) isolation of the L-amino acid.

A process for the production of L-amino acids, in particular L-threonine, in which the following steps are carried out:

Process for the production of L-amino acids using strains of the family enterobacteriaceae that contain an attenuated fruR gene

The invention relates to a process for the preparation of L-amino acids by the fermentation of recombinant microorganisms of the family Enterobacteriaceae. The microorganisms produce the desired L-amino acid and overexpress theoryibD gene encoding the putative glycosyl transferase enzyme. The microorganisms are cultivated in a medium under conditions in which the desired L-amino acid is enriched in the medium or in the cells. The amino acid is then isolated, optionally with constituents of the fermentation broth, and/or all or part (&#x2267;0 to 100%) of the biomass.

Process for L-amino acid production using enterobacteriaceae strains with enhanced yibD

This invention relates to a process for the production of L-amino acids, in particular L-threonine, in which the following steps are performed: a) fermentation of microorganisms of the Enterobacteriaceae family which produce the desired L-amino acid, in which at least one or more of the genes, selected from the group iclR and fadR, or nucleotide sequences coding therefor, are enhanced, in particular overexpressed, b) accumulation of the desired L-amino acid in the medium or in the cells of the bacteria and c) isolation of the desired L-amino acid.

Process for the production of L-amino acids using strains of the Enterobacteriaceae family which contain an enhanced fadR or iclR gene

The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

The invention relates to a process for the preparation of L-amino acids, in particular L-threonine, in which the following steps are carried out: a) fermentation of microorganisms of the Enterobacteriaceae family which produce the desired L-amino acid and in which at least one or more of the genes chosen from the group consisting of sucC and sucD, or nucleotide sequences which code for these, is or are enhanced, in particular over-expressed, b) concentration of the desired L-amino acid in the medium or in the cells of the bacteria, and c) isolation of the desired L-amino acid.

Process for the preparation of L-amino acids using strains of the enterobacteriaceae family which contain an enhanced sucC or sucD gene

The invention relates to a process for the preparation of L-amino acids, in particular L-threonine, which comprises overexpressing a ptsG gene coding for the glucose-specific IIBC component of the phosphotransferase system in Enterobacteriaceae.

Process for L-amino acid production using enterobacteriaceae strain with enhanced ptsG expression

The invention relates to amino acid-producing bacteria from the family Enterobacteriaceae, in particular from the species, which contain a stop codon chosen from the group amber, ochre and opal in the nucleotide sequence for the coding region of the rpoS gene and a suppressor for a stop codon chosen from the group amber suppressor, ochre suppressor and opal suppressor. This invention also relates to a process for preparing amino acids, in particular L-threonine, using these bacteria.

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Bielefeld, Germany

Mechthild Rieping