Oregon Health & Science University

Meaghan Hancock

Louis Picker

Scott G Hansen

Klaus Frueh

Jay A Nelson

Jay Nelson

Jonah Sacha

Daniel Malouli

CMV vectors that lack active UL128, UL130, UL146 and UL147 proteins that may also comprise one or more microRNA regulatory elements (MRE) that restrict expression of the CMV are provided. Immunization with CMV vectors having the described features allows selection of different CD8+ T cell responses&#x2014;CD8+ T cells restricted by MHC-Ia, MHC-II, or by MHC-E.

Cytomegalovirus vectors eliciting T cells restricted by major histocompatibility complex E molecules

Disclosed herein are recombinant CMV vectors comprising heterologous antigens and microRNA recognition elements to silence expression of CMV genes in the presence of microRNA derived from myeloid cells, an active UL128 protein and an active UL130 protein. Also disclosed are recombinant CMV vectors comprising heterologous antigens and microRNA recognition elements to silence expression of CMV genes in the presence of microRNA derived from myeloid cells, an inactive UL128 protein and an inactive UL130 protein. Also disclosed are methods of generating an unconventional immune response using these vectors. Such an immune response is characterized by generation of a CD8+ T cell response that is predominantly restricted by MHC-II.

CMV vectors comprising microRNA recognition elements

Disclosed are CMV vectors that lack active UL128, UL130, UL146 and UL147 proteins that may also comprise one or more microRNA regulatory elements (MRE) that restrict expression of the CMV. Immunization with the disclosed CMV vectors allow selection of different CD8+ T cell responses&#x2014;CD8+ T cells restricted by MHC-Ia, MHC-II, or by MHC-E.

Growing community of inventors

Aloha, OR, United States of America

Meaghan Hancock