Japan Science and Technology Agency

Arkray, Inc.

Mayumi Yamada

Yuka Shimomura

Itaru Imayoshi

Shinji Nagasaki

The present invention provides a photoactivatable Tet-OFF/ON system that can precisely control temporal and spatial gene expression. The present invention is a PA-Tet-OFF/ON system that includes a target gene expression cassette including a TRE having a TetO sequence, a promoter which is controlled by the TRE, and a target gene whose expression is controlled by the promoter; a first fusion protein expression cassette containing a gene which encodes a first fusion protein containing TetR or rTetR and a first protein; and a second fusion protein expression cassette containing a gene which encodes a second fusion protein containing p65AD and a second protein, in which the first protein and the second protein bind to each other to form a heterodimer only in a state of being irradiated with light at a specific wavelength.

Photoactivatable Tet expression control system

A method for recovering a metal, capable of recovering a metal easily without requiring the use of an organic medium, is provided. A complex between a chelating agent and a metal present in a sample is formed in a mixture prepared by mixing the chelating agent and the sample under pH conditions where the chelating agent can be insoluble in an aqueous medium. Then, the complex is recovered from the mixture, and further, the metal is recovered by dissolving the recovered complex in an aqueous medium under pH conditions that are different from the pH conditions where the chelating agent can be insoluble in an aqueous medium. By this method, a metal can be recovered easily without requiring the use of the use of an organic medium.

Growing community of inventors

Kyoto, Japan

Mayumi Yamada