Toyo Kohan Co., Ltd.

Yamaguchi University

Mayuko Hosoya

Masaaki Oka

Shoichi Hazama

Ryouichi Tsunedomi

Shuichi Kamei

A buffer composition for hybridization of a target nucleic acid is provided. The nucleic acid can include a nucleotide to be detected with a nucleic acid probe. The probe can contain a nucleotide sequence complementary to the target nucleic acid. The buffer can include a blocking nucleic acid having a nucleotide sequence complementary to a non-target nucleic acid having a nucleotide not to be detected corresponding to the nucleotide to be detected. The buffer composition can suppress non-specific hybridization to the nucleic acid probe even when a non-target nucleic acid is present. The use of the buffer composition can achieve excellent detection efficiency of the target nucleic acid.

Growing community of inventors

Kudamatsu, Japan

Mayuko Hosoya