National Institutes of Health, a Component of the US Dept. of Health & Human Services

Mayda Gursel

Dennis M Klinman

Daniela Verthelyi

Ihsan Gursel

Rainald Zeuner

James J Mond

Ken Ishii

The present disclosure relates to oligodeoxynucleotides that suppress an immune response. Methods are disclosed for preventing or treating an immune-mediated disorder, such as, but not limited to, an autoimmune disease, by administering a therapeutically effective amount of a suppressive oligodeoxynucleotide. Also disclosed are methods of suppressing an immune response in a subject by administering a therapeutically effective amount of a suppressive oligodeoxynucleotide.

Suppressors of CpG oligonucleotides and methods of use

The present disclosure relates to oligodeoxynucleotides that suppress an immune response. Methods are disclosed for preventing or treating inflammatory arthropathies by administering a therapeutically effective amount of a suppressive oligodeoxynucleotide.

Method of treating inflammatory arthropathies with supressors of CPG oligonucleotides

It is disclosed herein that agents that affect the activity and/or expression of CXCL16 can be used to alter the uptake of D-type CpG oligodeoxynucleotides (D ODNs). Methods of inducing an immune response are disclosed that include administering agents that increase the activity and/or expression of CXCL16 and a D ODN. Methods of decreasing an immune response to a CpG ODN are also disclosed. These methods include administering an agent that decreases the activity and/or expression of CXCL16. Compositions including one or more D-type ODNs and an agent that modulates that activity and/or expression of CXCL16 are provided.

Methods of altering an immune response induced by CpG oligodeoxynucleotides

Method of treating inflammatory arthropathies with suppressors of CpG oligonucleotides

D type CpG oligodeoxynucleotides are provided herein that include a sequence represented by the following formula:5'XXXPuPyCpGPuPyXXX(W)(G)-3&#x2032;wherein the central CpG motif is unmethylated, Pu is a purine nucleotide, Py is a pyrimidine nucleotide, X and W are any nucleotide, M is any integer from 0 to 10, and N is any integer from 4 to 10. Methods of using these oligodeoxynucleotides to induce an immune response are provided.

Oligodeoxynucleotide and its use to induce an immune response

Novel methods of rapidly generating dendrtic cells are disclosed herein. The methods include contacting a dendritic cell precursor with a D ODN to generate a mature dendritic cell. In one specific, non-limiting example, the method includes contacting the dendritic cell precursor or the mature dendritic cell with an antigen. The methods are of use both in vitro and in vivo.

Method for rapid generation of mature dendritic cells

D type CpG oligodeoxynucleotides are provided herein that include a sequence represented by the following formula:5' XXXPuPyCpG PuPyXXX(W)(G)-3&#x2032;wherein the central CpG motif is unmethylated, Pu is a purine nucleotide, Py is a pyrimidine nucleotide, X and W are any nucleotide, M is any integer from 0 to 10, and N is any integer from 4 to 10. Methods of using these oligodeoxynucleotides to induce an immune response are provided.

Novel methods for rapidly generating dendritic cells are disclosed herein. The methods include contacting a dendritic cell precursor with a D ODN to generate a mature dendritic cell. In one specific, non/limiting example, the method includes contacting the dendritic cell precursor or the mature dendritic cell with an antigen. The methods are of use both in vitro and in vivo.

D type CpG oligodeoxynucleotides are provided herein that include a sequence represented by the following formula:5'XXXPuPyCpG PuPyXXX(W)(G)-3&#x2032;wherein the central CpG motif is unmethylated, Pu is a purine nucleotide, Py is a pyrimidine nucleotide, X and W are any nucleotide, M is any integer from 0 to 10, and N is any integer from 4 to 10. Methods of using these oligodeoxynucleotides to induce an immune response are provided.

Growing community of inventors

Rockville, MD, United States of America

Mayda Gursel