Biocon Limited

Mayank Kumar Garg

Harish Iyer

Anuj Goel

Sanjay Tiwari

Mukesh Babuappa Patale

Chittnalli Ramegowda Naveen Kumar

Sulekha Joshi

Bimal Kumar

Saurabh Garg

Shrikumar Suryanarayan

Anand Khedkar

Partha Hazra

Nitesh Dave

Nita Roy

Goldy Sachdev

Gautam Krishnan

H S Manjunath

Govindasamy Manikam

The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogs, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as

Fermentation medias and processes thereof

The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogues, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as

The invention claims a process for making an insulin-oligomer conjugate IN-105. IN-105 precursor having formula G-A-V-R-[B-Chain]-R-D-A-D-D-R-[A-Chain] is cloned and expressed in. The biosynthetic precursor is then conjugated with an activated oligomer. The IN-105 precursor-oligomer conjugate is then treated with protease and purified to afford active insulin-oligomer conjugate of formula insulin-OC&#x2014;CH&#x2014;CH&#x2014;(OCHCH)&#x2014;OCH.

Process for the preparation of insulin conjugate IN-105

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Uttar Pradesh, India

Mayank Kumar Garg