Illumina Corporation

Illumina Cambridge Limited

Illumina Singapore Pte. Ltd.

Maxwell Zimmerley

Hai Quang Tran

Randall Smith

Lewis J Kraft

Brian Douglas Mather

Steven Modiano

M Shane Bowen

Wayne N George

Dajun Yuan

Alexandre Richez

Sean M Ramirez

Xavier Von Hatten

Liangliang Qiang

Ludovic Vincent

Hong Xie

Gianluca Andrea Artioli

Julia Morrison

Arthur Pitera

Wei Wei

Edwin Li

Hayden Black

Krystal Sly

Innsu Daniel Kim

Sojeong Moon

Ryan Sanford

Silke Musa

Sara Bakhshi

Gerald Kreindl

Liangliang Quang

Arthur J Pitera

In one example, a method of preparing a fluidic channel includes covalently coupling a first region of a substrate to a first region of a cover using first moieties covalently coupled to the first region of the substrate and second moieties covalently coupled to the first region of the cover. The covalent coupling between the first region of the substrate and the first region of the cover suspends a second region of the cover over a second region of the substrate to form a fluidic channel.

Fluidic devices including fluidic channels, and methods of making the same

An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.

Interposer with first and second adhesive layers

An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

Protective surface coatings for flow cells

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San Diego, CA, United States of America

Maxwell Zimmerley