Institut Pasteur

Maxime Mistretta

Giulia Manina

Multiplexable microfluidic culture chamber for imaging monolayer growth of single cells The present invention relates generally to a microfluidic device (), particularly for use in single cell analysis. More specifically, the present invention relates to a microfluidic device () comprising at least one chamber (), in particular at least two chambers () comprising a deformable membrane () and having a structure and geometry configured to enable formation of two-dimensional cell culture, in particular two-dimensional cell growth area (), and imaging thereof over a growth period or a time period sufficient to analyze cells, in particular to monitor cell growth. The microfluidic device () allows for multi-condition operation of single-cell screening at high spatiotemporal resolution. The present invention also relates to methods for fabrication and use of such devices.

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PARIS, France

Maxime Mistretta