Massachusetts Institute of Technology

Harvard College

The Brigham and Women's Hospital, Inc.

The Broad Institute, Inc.

Max Walt Shen

David R Liu

David Kenneth Gifford

Richard Irving Sherwood

Jonathan Yee-Ting Hsu

Mandana Arbab

The specification provides methods for introducing a desired genetic change in a nucleotide sequence using a double-strand break (DSB)-inducing genome editing system, the method comprising: identifying one or more available cut sites in a nucleotide sequence; analyzing the nucleotide sequence and available cut sites with a computational model to identify the optimal cut site for introducing the desired genetic change into the nucleotide sequence; and contacting the nucleotide sequence with a DSB-inducing genome editing system, thereby introducing the desired genetic change in the nucleotide sequence at the cut site.

Systems and methods for predicting repair outcomes in genetic engineering

Growing community of inventors

Cambridge, MA, United States of America

Max Walt Shen