Carl Zeiss Microscopy Gmbh

Fraunhofer-gesellschaft Zur Foerderung Der Angewandten Forschung E.v.

4d Photonics Gmbh

Max Funck

Thomas Milde

Johannes Winterot

Toufic Jabbour

Lars Stoppe

Ingo Fahlbusch

Johannes Knoblich

Georg Herbst

Uwe Wolf

Benno Radt

Hans Tandler

Alexander Gaiduk

Jan Buchheister

Andrea Berner

Dominik Stehr

Jan Dolkemeyer

Markus Gnauck

Wolf Jockusch

Enrico Geißler

Oliver Bruchwald

Christoph Franz

Michael Strotkamp

Jochen Stollenwerk

The invention relates to a method of correcting illumination-dependent aberrations in a modular digital microscope comprising a coaxial bright field illumination apparatus, a motor-driven zoom apparatus, an objective, a digital image acquisition unit and an image processing unit. The method according to the invention is based on the use of calibration data, which are preferably stored in the image processing unit as a calibration data record. The calibration data record is established for each combination of objective and zoom and stored in a data memory. The method is integrated into the image processing unit for live-correction or available as software for image post-processing of microscope images. The invention further relates to a digital microscope.

Method for correcting illumination-dependent aberrations in a modular digital microscope, digital microscope and data-processing program

For image recording purposes, an object is illuminated at a plurality of illumination angles. A detector captures a plurality of images (-) of the object for the plurality of illumination angles. An electronic evaluating device applies an image correction to at least some of the plurality of images (-), said image correction comprising a displacement (T, T), wherein the displacement (T, T) depends on the illumination angle () used when recording the respective image (-). The corrected images (-) may be combined.

Image capturing device and method for image capturing

The invention relates to a method for calibrating a digital optical imaging system, comprising at least one motorized or coded zoom system and an image sensor, to a method for correcting aberrations in such an imaging system, and to an optical imaging system which is configured to carry out the methods according to the invention. During the calibration method, a reference object is recorded in various zoom settings and the image is corrected pixel-wise with digital-optical means using a previously determined model. To this end, distortion correction coefficients and image stability correction coefficients are ascertained. The real total magnification of the system is ascertained from the corrected image. The model ascertained in the calibration process also serves for correcting aberrations during operation of the imaging system.

Method for calibrating a digital optical imaging system having a zoom system, method for correcting aberrations in a digital optical imaging system having a zoom system, and digital optical imaging system

The invention relates to a method for calibrating an optical instrument which comprises at least a motorized zoom system, an objective, an image sensor and an image processing unit. The method comprises the following steps: establishing calibration data Dof the zoom system with a reference objective and storing these in an internal memory of the zoom system; establishing calibration data Dof the objective with a reference zoom system and storing these in an internal memory of the objective; reading the internal memories of the zoom system and of the objective and applying a digital-optical correction of an image acquired by an image sensor with the calibration data Dand D. The invention moreover relates to an optical instrument, in particular a digital microscope, to which the calibration method according to the invention can be applied.

Method for calibrating a digital optical instrument and digital optical instrument

The invention relates to a ring illumination device () for a microscope objective (), and a microscope objective () provided with such a ring illumination device (). The ring illumination device () has at least two LED units () which are accommodated in an illumination ring () which can be connected to the microscope objective (). According to the invention, the ring illumination device () has at least one lower entrance opening () and at least one upper outlet opening () for cooling air, for cooling the LED units ().

Ring illumination device for a microscope objective, and microscope objective

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Weimar, Germany

Max Funck