Ciba-geigy Corporation

Max Brugger

Bernhard Riniker

Werner Rittel

Peter Sieber

Bruno Kamber

Hendrik M Greven

Robert Neher

Friedrich Werner Kahnt

Herbert Zuber

The new hypocalcaemically active peptides of formula I ##STR1## and corresponding compounds in which one or more of the asparagine and glutamic acid radicals are replaced by the aspartic acid or glutamic acid radical and/or the aspartic acid radical is replaced by the asparagine radical, their dimers, especially those in which 2 identical peptide sequences (1-32 and 1'-32') are joined in an anti-parallel arrangement via the cysteine radicals 1,7' and 7,1' by means of a disulfide bond, and derivatives are useful as hypocalcaemic agents and are prepared by splitting off groups protecting at least one amino or one carboxyl group or oxidizing the corresponding sulfides to the disulfides or condensing together adequate peptides.

Peptides and process for their manufacture

Synthetic hypocalcaemic dotriacontapeptide of the formula

Hypocalcaemic peptides and processes for their manufacture

Peptides of the calcitonin type when obtained by synthesis can be purified in a simple manner by forming inner salts, which precipitate from aqueous solutions of salts of the peptides with acids or bases when these solutions are adjusted to the isoelectric range. Impurities remain in solution, while the inner salts separate practically quantitatively. The precipitation is completed after several hours. The peptides can then be recuperated by treatment with an acid, especially a therapeutically suitable acid. The process affords products which are superior to those obtained by purification methods hitherto known and gives higher yields. Other long-chain peptides such as ACTH, insulin or glucagon cannot be purified in this manner.

Process for the purification of synthetic calcitonins

The new hypocalcaemically active peptides of formula I

Growing community of inventors

Birsfelden, Switzerland

Max Brugger