Politecnico Di Milano

Fondazione Istituto Italiano Di Tecnologia

Universitá Degli Studi Di Genova

Micro Photon Devices Srl

Mauro Buttafava

Franco Zappa

Alberto Diaspro

Giuseppe Vicidomini

Paolo Bianchini

Federica Villa

Paola Taroni

Alberto Tosi

Marco Castello

Gabriele Scrofani

Giorgio Tortarolo

Colin J R Sheppard

Federica Alberta Villa

Alessandro Torricelli

Davide Contini

Antonio Giovanni Pifferi

Laura Di Sieno

Enrico Conca

A method for operating a point laser-scanning microscope includes scanning a sample with a focused illumination laser beam; recording a plurality of images by detecting elements being configurable to an intensity mode, in which the recorded images are intensity images g(n) related to photons collected during an entire dwell time of the illumination beam on an individual position n, or to a time-resolved mode, in which the recorded images are time-resolved images g(n, t), the collected photons being discriminated based on their arrival times to individual detecting elements; calculating a fingerprint image a by summing the plurality of intensity images g(n) over all positions n; estimating shift matrices sand sfrom the intensity images g(n); reconstructing at least one of a time-resolved object function fand an intensity object function f; and visualizing at least one of a high-resolution time-resolved image fand a high-resolution intensity image f.

Time-resolved imaging method with high spatial resolution

Growing community of inventors

Sarmato, Italy

Mauro Buttafava