Universitá Degli Studi Di Padova

Biosil A.g.

Fondazione Città Della Speranza—onlus

Cryo-save Ag

Maurizio Muraca

Giovanni Carturan

Anna Maria Teti

Gianmarco Villano

Patrizia Pontisso

Mariagrazia Ruvoletto

Santina Quarta

Alfredo Cappariello

Renzo Dal Monte

Alessandra Biasiolo

Alessandra Fierabracci

The present invention concerns the therapeutic use of extracellular vesicles isolated from a genetically modified cell line over-expressing SerpinB3. In particular, said therapeutic use aimed at the treatment of acute pathologies characterized by ischemic or inflammatory tissue damage or by cell injury due to oxidative stress, such as cardiac, cerebral, intestinal, renal or limb ischaemia. Further examples of use consist of the preservation of transplant organs, including heart, lung, liver, bladder, pancreas and intestine. In a further aspect the invention relates to the use of a pharmaceutical composition comprising extracellular vesicles isolated from a genetically modified cell line over-expressing SerpinB3 and a pharmaceutically acceptable vehicle.

Extracellular vesicles isolated from genetically modified cells over-expressing SERPINB3 for use in medicine

The present invention relates to RANKL+ cellular vesicles isolated from osteoblastic lineage cells, optionally immortalised, their use for the therapeutic treatment of bone pathologies and for diagnostic purposes, and processes for the production of said vesicles.

Extracellular vesicles derived from osteoblastic lineage cells for therapeutic and diagnostic use

A suspension of animal cells is incubated with supports to adhere the cells to the supports. Preferably, the supports have pores that provide pore volume, and the cells are grown during incubation until most of the available pore volume is filled with cells. An encapsulating layer is then formed around the supported cells by exposing the cells on the supports to a reactive gas composed of a carrier gas such as sterile air saturated with an inorganic alkoxide followed by treatment with steam to hydrolyze residual alkoxide groups. The encapsulated cells are stored by immersion in culture media. The cells may be in the form of cell aggregates, and the supports can be sterilized. The supports and encapsulating layer can have pores of a size that permit free exchange nutrients and metabolic products, and excludes the cells from contacting antibodies or immune cells when implanted. The encapsulated cells can used in an extracorporeal device or implanted directly.

Encapsulation of supported animal cells using gas-phase inorganic alkoxides

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Padua, Italy

Maurizio Muraca