Quest Diagnostics Investments, Inc.

3m Innovative Properties Company

Diasorin S.p.a.

Focus Diagnostics, Inc.

Maurice Exner

Michelle M Tabb

Huong Mai

Edgar Ong

Michael Aye

Luca Jacky

Yin-Peng Chen

Lakshmi Nair

Heather Vincent

Peter D Ludowise

Kyle Armantrout

David A Whitman

Lucien A E Jacky

Amy Rogers

Jules Chen

Hasnah Hamdan

Michael Lewinski

Jamie L Platt

Emberlee Eleazar

Lucien AE Jacky

Kyle C Armantrout

Provided herein are methods for identifying the presence or absence of a target nucleic acid from a microorganism using direct amplification without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids.

Direct amplification and detection of viral and bacterial pathogens

Disclosed is a method for determining the presence ofcomplex nucleic acids in a biological sample. In particular, the mig gene ofand the DT1 gene ofare detected, preferably following amplification. In addition, the method distinguishes between species ofand. Also described are oligonucleotides that can be used as primers to amplify target genes such as mig and DT1 genes and as probes as well as kits containing the oligonucleotide.

Kits for detectingnucleic acid

Disclosed are methods of identifying a methicillin-resistant(MRSA) in a sample wherein the methods involve detecting a-specific nucleic acid sequence, mecA and mecC, in the sample. Kits for determining the presence of MRSA in a sample are also provided.

Detection of methicillin-resistantin biological samples

Disclosed is a method for determining the presence ofcomplex nucleic acids in a test sample. In particular, regions of the IS6110 preferential locus (ipl) 3'-flanking region of thecomplex genome are amplified and detected. In addition, oligonucleotides that can be used as primers to amplify the ipl 3&#x2032;-flanking region and probe oligonucleotides are described.

[object Object]

Systems and methods for processing sample processing devices. The system can include a sample processing device comprising a detection chamber, a motor configured to rotate the sample processing device about an axis of rotation, and an optical module operatively positioned relative to the sample processing device and configured to determine whether a selected volume of material is present in the detection chamber of the sample processing device. The method can include rotating the sample processing device about an axis of rotation, and determining whether a selected volume of material is present in the detection chamber, while rotating the sample processing device. In some embodiments, determining whether a selected volume of material is present can be performed by optically interrogating the detection chamber for an optical property of the material.

Systems and methods for detecting the presence of a selected volume of material in a sample processing device

Provided are methods for identifying the presence or absence of a target nucleic acid from a microorganism using direct amplification without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids. Further provided are reagent mixtures that allow for direct amplification of a sample, without the step of nucleic acid extraction.

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Mission Viejo, CA, United States of America

Maurice Exner