Geneohm Sciences Canada, Inc.

UniversitÈ Laval

Infectio Diagnostic (i.d.i.), Inc.

Genohm Sciences Canada, Inc.

Maurice Boissinot

Mario Leclerc

Michel G Bergeron

Marc Ouellette

Ann Huletsky

Paul H Roy

Francois J Picard

Hoang Anh Ho

François J Picard

Christian Ménard

Christian Menard

Jean Pierre Gayral

Martine Bastien

Hoang-Anh Ho

Hoang Ahn Ho

Jean-Pierre Gayral

François Picard

Provided herein are compositions and methods for the detection of

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Compositions and methods for the detection of vancomycin-resistant pathogens using primers and/or probes to the vanA and vanB genes.

Methods and probes for detecting a vancomycin resistance gene

An optical sensor for detecting a target comprising a singlestranded aptamer complementary to said target, and a water-soluble cationic polythiophene derivative of the following formula: wherein 'n' is an integer ranging from 6 to 100, is disclosed. The optical sensor allows for the detection of targets selected from the group consisting of potassium ions, small organic molecules, amino acids, proteins, whole cells and nucleotides. The detection is based on the formation of hybrid anionic aptamer/cationic poly-thiophene complexes.

Optical sensors based on hybrid aptamer/conjugated polymer complexes

This invention relates to reagent comprising: any one of cells, viral particles, organelles, parasites, cells comprising organelles, cells comprising viral particles, cells comprising parasites, cells comprising bacterial cells and any combination thereof, the cells, viral particles, organelles or parasites comprising at least one nucleic acid sequence serving as an internal control (IC) target for nucleic acid testing (NAT) assay; wherein the reagent is suitable to be added to a test sample undergoing sample preparation to release, concentrate and/or purify nucleic acids and amplification and/or detection of nucleic acids so as to be used to verify: (i) the efficiency of sample preparation; and (ii) the efficiency of nucleic acid amplification and/or detection. The present invention also relates to a method to verify or validate the preparation and amplification and/or detection of a nucleic acid target sequence in a sample spiked with a reagent of the present invention.

Biological reagents and methods to verify the efficiency of sample preparation and nucleic acid amplification and/or detection

Four highly conserved genes, encoding translation elongation factor Tu, translation elongation factor G, the catalytic subunit of proton-translocating ATPase and the RecA recombinase, are used to generate species-specific, genus-specific, family-specific, group-specific and universal nucleic acid probes and amplification primers to rapidly detect and identify algal, archaeal, bacterial, fungal and parasitical pathogens from clinical specimens for diagnosis. The detection of associated antimicrobial agents resistance and toxin genes are also under the scope of the present invention.

Highly conserved genes and their use to generate probes and primers for detection of microorganisms

Novel methods allowing for the simple optical and electrochemical detection of double-stranded oligonucleotides are disclosed. The methods are rapid, selective and versatile. Advantageously, they do not require any chemical reaction on the probes or on the analytes since they are based on different electrostatic interactions between cationic poly(3-alkoxy-4-methylthiophene) derivatives and single-stranded or double-stranded (hybridized) oligonucleotides.

Detection of negatively charged polymers using water-soluble, cationic, polythiophene derivatives

Novel methods allowing for the simple optical detection and electrochemical detection of doubled-straded oligonucleotides are disclosed. The methods are rapid, selective and versatile. Advantageously, they do not require any chemical reaction on the probes or on the analytes since they are based on different electrostatic interactions between cationic poly(3-alkoxy-4-methylthiophene) derivatives and single-stranded or doubled-stranded (hybridized) oligonucleotides.

Novel methods allowing for the simple optical and electrochemical detection of double-stranded oligonucleotides are disclosed. The methods are rapid, selective and versatile. Advantageously, they do not require any chemical reaction on the probes or on the analytes since they are based on different electrostatic interactions between cationic poly (3-alkoxy-4-methylthiophene) derivatives and single-stranded or double-stranded (hibridized) oligonucleotides.

Growing community of inventors

Saint-Augustin-de-Desmaures, Canada

Maurice Boissinot