Monsanto Company

Harvard College

Mau-Jung Kuo

Joseph Feder

William R Tolbert

Moses J Folkman

Charles Lewis, Jr

Margaret J Reents

Katharine Ku

Robert E Miller

Process for the production of human angiogenic factor in vitro comprising growing the human diploid cell line IMR-90 on support surface in nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate angiogenic factor and recovering the resulting angiogenic factor from the cells or cell product.

Production of angiogenic factor by cell culture

A method of submerged cell culture is provided which employs a microcarrier support comprising cross-linked polystyrene resin beads derivatized with amino acids, peptides, or hydroxy carboxylic acids.

Method of culturing microcarrier supported cells

Process for the production of human TAF in vitro comprising growing the human colon adenocarcinoma cell line HT-29 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Production of tumor angiogenesis factor by cell culture

Process for the production of human TAF in vitro comprising growing the human Burkitt lymphoma cell line Raji in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Process for the production of human TAF in vitro comprising growing the human medulloblastoma cell line TE-671 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Process for the production of human TAF in vitro comprising growing the human melanoma cell line A-375 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Process for the production of human TAF in vitro comprising growing the human rhabdomyosarcoma cell line RD in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Process for the production of human TAF in vitro comprising growing the human liver adenocardinoma cell line SK-HEP-1 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

The yield of urokinase produced by cell culture of kidney cells is improved by incorporating in the cell culture medium an elevated level of phenylalanine.

Production of urokinase

A cell culture reactor system is provided for the growth of cells in vitro which employs elongate hollow or solid fibers arranged in a shallow layer configuration as a matrix for cell attachment in which the flow path of the culture media is directed substantially uniformly through the fiber layer and substantially transverse to the plane of the elongate axes of the fibers.

Growing community of inventors

Creve Coeur, MO, United States of America

Mau-Jung Kuo