Ge Healthcare Bio-sciences Ab

Ge Healthcare Bioprocess R&d Ab

Ge Healthcare UK Limited

Cytiva Bioprocess R&d Ab

Mattias Algotsson

Ronnie Palmgren

Jean-Luc Maloisel

Lars C Andersson

Eggert Brekkan

Jimmy Hedin Dahlstrom

Lee Hedemyr

Nils Norrman

Jinyu Zou

Nicolas Thevenin

Bengt Bjellqvist

Philippe Busson

Barry Johnson

Ylva Laurin

Miles W Burrows

Stefan D Eriksson

Philip Robert Mallender

Douglas Mark Green

Sarah Janet Clegg

Jimmy Hedin Dahlström

Barry Edmund Johnson

Åsa Bjurling

The present invention relates to a method to improve chromatography beads. More closely, the invention relates to a novel method for production of dextran-containing porous media and chromatography media produced with this method. In the method, the chromatography media is subjected to dextranase-treatment leading to improved pressure-flow properties of the media.

Method and chromatography medium

The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.

Chelator and use thereof

The invention discloses a cellulose or glass fiber paper for preservation of biological samples, which comprises 4-30 wt % of a hydrophilic branched carbohydrate polymer. It also discloses a method for preservation of biological samples by applying and drying them on the paper.

Sample preservation method and sample preservation substrate

The invention discloses a sample preservation method which comprises the following steps: a) providing a paper substrate comprising ligands, where the ligands comprise charged groups, b) applying a sample comprising at least one analyte and at least one contaminant on the paper substrate, c) drying the sample on the paper substrate and, d) extracting at least part of the paper substrate to provide a solution of the analyte.

Sample preservation method and sample application substrate

The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity. The dimeric pentadentate chelator according to the invention has the following general formula:  

The present invention relates to a method for synthesis of an acrylamide derivative, starting with dissolving a salt of a nucleophilic amine in water to form an aqueous solution and desalting said solution with a base, comprising the following steps:

Method for synthesis of acrylamide derivatives

The invention relates to an acrylic monomer defined by formula (I): wherein The invention also relates to a polymer comprised of repeated such monomer units as well as a separation matrix prepared thereof.

Monomers useful in the preparation of separation matrices

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Uppsala, Sweden

Mattias Algotsson