Harvard College

Albert Einstein College of Medicine

Whitehead Institute for Biomedical Research

Stichting Het Nederlands Kanker Instituut-antoni Van Leeuwenhoek Ziekenhuis

Academisch Ziekenhuis Leiden (h.o.d.n. Lumc)

Matthijs Raaben

Thijn Reinout Brummelkamp

Kartik Chandran

Jan E Carette

Lucas Tilmann Jae

Sean Whelan

Jeannette Henrica Wilhelmina Leusen

Antonius Nicolaas Maria Schumacher

Joppe Daniël Maria Nieuwenhuis

Markus Brockmann

Vincent Arthur Blomen

Joppe Daniel Nieuwenhuis

The present invention comprises generally applicable methods for identifying endogenous physiologically relevant genetic elements that affects a intracellular phenotype of interest. In the methods, non-living cells that have been subjected to a mutagenesis treatment are sorted based on phenotype and analyzed to identify the genetic element. By use of these methods, elements previously unknown to be involved in a phenotype can be identified, for example in relationship to health conditions, external stress or drug response, in particular in cancer.

Assays to identify genetic elements affecting phenotype

Methods and assays for treating a subject with a filovirus infection using an agent that inhibits Niemann-Pick CI (NPCI), VPSII, VPSI6, VPSI8, VPS33A, VPS39, VPS41, BLOCISI, BLOCIS2, GNPTAB, PIKFYVE, ARHGAP23 or FIG4. Methods for screening for an agent that treats and/or prevents infection of a subject with a filovirus, where the methods comprise determining whether the agent inhibits one or more of Niemann-Pick CI (NPCI), VPSII, VPSI6, VPSI8, VPS33A, VPS39, VPS41. BLOCISI, BLOCIS2, GNPTAB, PIKFYVE, ARHGAP23 or FIG4, wherein an agent that inhibits one or more of NPCI, VPSII, VPSI6, VPSI8, VPS33A, VPS39, VPS41, BLOCISI, BLOCIS2, GNPTAB, PIKFYVE, ARHGAP23 or FIG4 is a candidate for treating and/or preventing an infection with a filovirus and wherein an agent that does not inhibit NPCI, VPSII, VPSI6, VPSI8, VPS33A. VPS39, VPS41, BLOCISI, BLOCIS2, GNPTAB, PIKFYVE, ARHGAP23 or FIG4 is not a candidate for treating and/or preventing an infection with a filovirus.

Methods and assays for treating filoviridae infections

Growing community of inventors

Amsterdam, Netherlands

Matthijs Raaben