Ares Trading S.a.

Sartorius Stedim Biotech Gmbh

Fresenius Kabi Deutschland Gmbh

Matthieu Stettler

Martin Jordan

Hervé Broly

Thomas Solacroup

Herve Broly

Oscar-Werner Reif

Reinhard Baumfalk

Stefan Obermann

David Bruhlmann

Florian Wurm

Yolande Rouiller

Anais Muhr

Maria De Jesus

Arnaud Perilleux

Anaïs Muhr

The present invention relates to a method of maintaining cell viability at a level of at least 80% for up to 14 days in a fed-batch culture of cells producing a recombinant protein, the method comprising maintaining the cell culture medium at a pH of 6.5 to 7.0 and at a temperature of 31&#xb0; C. to 35&#xb0; C. for at least 5 consecutive days of the duration of the cell culture method.

Cell culture methods for increased cell viability

The present invention relates to a method of increasing biosimilarity of a TNF &#x3b1; binding protein to a reference TNF &#x3b1; binding protein by modifying the charge profile of the binding protein produced by cell culture in a cell culture medium, by making an adjustment to a cell culture condition The adjustment is an increase in the concentration of a metal ion in the cell culture medium and the adjustment occurs on one or more days during the cell culture method, whereby the adjustment in the cell culture condition results in the modification of the charge profile of the binding protein. The present invention also relates to a biosimilar protein obtainable by such a process.

Methods for increasing biosimilarity

The invention is in the field of cell culture. Particularly the invention relates to methods of culturing a host cell expressing a recombinant protein in a cell culture medium comprising an effective amount of a triazine dye or supplemented with an effective amount of a triazine dye, whereby production of said protein is increased relative to cells grown without said triazine dye or any other inducer.

Methods for modulating production profiles of recombinant proteins

The invention is in the field of cell culture. Particularly the invention relates to methods of culturing a host cell expressing a recombinant protein in a cell culture medium comprising an effective amount of 4,4'diisothiocyanostilbene-2,2&#x2032;-disulfonic acid (DIDS) or supplemented with an effective amount of DIDS, whereby production of said protein is increased relative to cells grown without DIDS.

The present invention relates to a method of reducing serine for asparagine misincorporation in a protein produced by a culture of cells in a cell culture method, the method comprising the addition of asparagine and iron to the cell culture medium.

Method of reducing serine for asparagine misincorporation

The present invention relates to a cell culture method for adjusting the proportion of G0, G1, G2, and high-mannose galactosylation variants in a population of a recombinant protein produced by a culture of cells in a cell culture medium by supplementing the cell culture medium with manganese at one or more days of the cell culture method duration.

Methods for modifying glycosylation using manganese

The present invention relates to new serum- and protein-free culture media. These media are high performance culture media, which notably improve mammalian fed-batch cultures. The present invention also relates to methods for preparing and/or designing the medium, and methods of use thereof.

Medium for high performance mammalian fed-batch cultures

The invention relates to a device for detecting a sample in a longitudinal sample container (), comprising a sample container holder () for holding the sample container in a housing. Said sample container holder comprises a side viewing window in the region of at least one longitudinal side of the sample container, and a front viewing window () in the region at least one front side of the sample container. Said device also comprises a first illumination arrangement for illuminating the sample container through the front viewing window, a second illumination arrangement for illuminating the sample container through the side viewing window, and an imaging photodetector for detecting a first image of the sample illuminated by means of the first illumination arrangement, and a second image of the sample illuminated by means of the second illumination arrangement. Said detection takes place through the side viewing window. Said device further comprises a digital data processing unit for overlaying the first and second images and for representing the resulting overlay image on a display device connected to the data processing unit.

Method and detection device for the imaging detection of a sample

The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: coloring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are colored to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the colored cells is centrifuged as a sample in a sample vessel () until the solid content present in the sample has settled as a compressed cell cake (), and, in order to determine the proportions, the volumes of differently colored sections () of the compressed cell cake () are determined as a measure of the proportions of cells of different viability.

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Vucherens, Switzerland

Matthieu Stettler