Epigenomics Ag

Hoechst Aktiengesellschaft

Carl Freudenberg Kg

Matthias Schuster

Reimo Tetzner

Fabian Model

Andrew Z Sledziewski

Juergen Distler

Catherine E Lofton-Day

Ralf Lesche

Thomas Hildmann

Xiaoling Song

Kurt Berlin

Dimo Dietrich

Matthias Ballhause

Ulrich Schneider

Jörn Lewin

Antje Kluth Lukas

Ute Wagner

Reinhold Wasserkort

Marco Sutter

Hans-Dieter Jakubke

Gerald Jarre

Iain Smith

Michael Krouse

Shannon Payne

Jürgen Distler

Cathy E Lofton-Day

Antje Kluth

Heike Ziebarth

Dominic Kramer

Nermina Zaplatilek

Theo De Vos

Theo Devos

David Gütig

Joe Lograsso

Jennifer Maas

Aavo Aaviksaar

Volker Liebenberg

Jesse Ho

Thomas Schlegel

Andrew M Morotti

Cathy Lofton-Day

Reinhold Wassenkort

The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.

Methods and nucleic acids for analyses of cellular proliferative disorders

Aspects of the present invention relate to compositions and methods for providing DNA fragments from a remote sample. In particular aspects a remote sample comprising DNA is provided, DNA is isolated from the remote sample, and the isolated DNA is treated in a way which allows differentiation of methylated and unmethylated cytosine. Additional, particular embodiments provide compositions and methods for methylation analysis of DNA derived from a remote sample. Other aspects provide for compositions and methods of whole genome amplification of bisulfite treated DNA. Other aspects provide methods for determining the presence or absence of methylation of at least one cytosine, or a series of cytosines in cis, in human DNA of a blood sample, a plasma sample, a serum sample or a urine sample from a human individual.

Method for determining the presence or absence of methylation in a sample

A textile fabric for preventing the penetration and water spreading in cables, having at least one layer, which is at least partially covered by an absorbent material and has pores, which pores can be at least partially closed under the effect of liquid due to absorbent material swelling, the absorbent material being bonded to the textile layer, at least in some areas, has a DIN ISO 9073-3 tensile strength in machine direction of &#x3e;50 N/5 cm, and obtainable by a method involving: treating a layer containing pores with a mixture containing a polymerizable monomer or oligomer and a cross-linking agent and, as absorbent material precursor, a wetting agent and initiator, and polymerization of the monomer or oligomer under formation of a bonded connection between the absorbent material and the layer. The textile fabric can have a DIN EN ISO 9237 air permeability in dry state of greater than 200 dm/(ms).

Textile fabric for preventing the penetration and the spreading of water in cables

Methods and nucleic acids for the analyses of cellular proliferative disorders

Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc.). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, and are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable.

Method for providing DNA fragments derived from an archived sample

Aspects of the invention provide methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. Particular aspects disclose and provide genomic sequences the methylation patterns of which have substantial utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.

Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, and are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable.

Aspects of the invention relate to composition and methods for the providing of DNA for methylation analysis that is in particular suitable to be applied in reference laboratories. Further 5 aspects of the invention relate to composition and methods for the highly specific and sensitive methylation analysis of the Septin 9 gene also in particular suitable to be applied in reference laboratories.

Method for methylation analysis

Methods and nucleic acids for analysis of cellular proliferative disorders

Process for preparing peptides

Growing community of inventors

Berlin, Germany

Matthias Schuster