Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Matthias Liebergesell

Kanwarpal Singh Dhugga

George William Singletary

Ramesh V Nair

Xiaoming Bao

David A Selinger

Deborah J Wetterberg

Scott E Nichols

Chun Ping Li

Jian G Dong

William Dean Hitz

Jinrui Shi

Kristen Briggs

Gary A Huffman

Carl W Garnaat

Jerome P Ranch

Patrica Lynne Fallis

Ramesh Nair

The present disclosure concerns methods and compositions relating to UXS polypeptides and/or nucleic acids encoding UXS polypeptides. In certain claims, the methods and compositions are of use to improve digestibility and/or ease of grain processing. Such improvements relate to a modulation in arabinoxylan and/or hemicellulose content in transgenic plants. Such plants can, for example, comprise one or more nucleic acid sequences that inhibit expression of one or more UDP-Xylose Synthase (USX) genes.

UDP-xylose synthases (UXS) polynucleotides, polypeptides and uses thereof

Auxotrophicand methods employing auxotrophicare provided. Auxotrophicmay be used in a variety of methods including biologically containingcomprising a transgene and transforming a plant cell without using ancounter-selective agent. Transforming maize immature embryos using anauxotrophic for thymidine results in comparable transformation efficiency as transformation achieved using prototrophic. Methods for producing anthymidine auxotroph and using the auxotroph in transformation methods are disclosed. Transformed tissues and plants produced using methods of the present invention are also provided.

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The present disclosure concerns methods and compositions relating to UXS polypeptides and/or nucleic acids encoding UXS polypeptides. In certain claims, the methods and compositions are of use to improve digestibility and/or ease of grain processing. Such improvements relate to a modulation in arabinoxylan and/or hemicellulose content in transgenic plants. Such plants can, for example, comprise one or more nucleic acid sequences that inhibit expression of one or more UDP-Xylose Synthase (UXS) genes.

UDP-xylose synthases (UXS) polynucleotides, polypeptides, and uses thereof

Compositions and methods for modulating MIOX activity are provided. Such compositions include nucleotide sequences for novel MIOX sequences obtained from maize, amino acid sequences for the proteins encoded by the nucleotide sequences of the invention, and variants and fragments thereof. Methods of the invention involve introducing into a plant a nucleotide construct comprising a MIOX nucleotide sequence operably linked to a promoter that drives expression in a plant. Expression of the novel nucleotide sequences disclosed herein confers advantageous agronomic properties on a plant. Transformed plants, plant cells, and seeds are additionally provided.

Modulating myo-inositol catabolism in plants

The invention relates to the genetic manipulation of plants to produce polyhydroxyalkanoate, particularly in the peroxisomes. Methods for producing such polymers in plants and host cells are provided. Such methods find use in producing biodegradable thermoplastics in plants and other organisms. Nucleotide molecules, expression cassettes, and genetically manipulated host cell, plants, plant tissues, and seeds are also provided.

Production of polyhydroxyalkanoate in plants

The invention relates to the genetic manipulation of plants to produce polyhydroxyalkanoate, particularly in the peroxisomes. Methods for producing such polymers in plants and host cells are provided. Such methods find use in producing biodegradable thermoplastics in plants and other organisms. Nucleotide molecules, expression cassettes, and genetically manipulated host cells, plants, plant tissues, and seeds are also provided.

The invention relates to genes encoding polyhydroxyalkanoate synthases. Compositions and methods for producing polyhydroxyalkanoate are provided. Such compositions and methods find use in producing biodegradable thermoplastics in host cells and transgenic plants. Isolated nucleotide molecules, isolated polypeptides, expression cassettes and genetically manipulated host cells, plants, plant tissues, plant cells and seeds are also provided.

Growing community of inventors

West Des Moines, IA, United States of America

Matthias Liebergesell