Gen-Probe, Inc.

Matthias Jost

Daniel P Kolk

Patrick Lynn Peterson

Tony Luu

Mehrdad R Majlessi

Daniel Kolk

Ankur Shah

Donald A Martin

Pamela Douglass

Paul M Darby

Barbara Lynn Eaton

Siobhan Miick

Steven Mauch

JoAnn Jackson

Matthew Brentnall

Robert Stephen Dickson

Mark Filipowsky

Vladislav Nodelman

Cheryl Lynn Crowell

Donald Martin

Joann Jackson

Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. A biological sample is combined with an alkaline composition that lyses cells and denatures DNA to create a first liquid composition. The first liquid composition is then mixed with a buffer, a detergent, and a solid support that captures DNA to create a second liquid composition. The buffer, detergent, and solid support can be delivered as components of a single reagent. Captured DNA strands can be used as templates in subsequently performed nucleic acid amplification and detection reactions with improved sensitivity.

Sample preparation method and system

The disclosure provides dried compositions providing reagents for nucleic acid amplification, which are essentially free of inorganic salts. Lack of inorganic salts increases stability of such compositions and decreases formation of byproducts. Salts required for use of enzymes in the composition are supplied on reconstitution.

Dried amplification compositions

This disclosure concerns amplification primers, hybridization assay probes, compositions containing such primers and probes, and associated reagents, kits, and methods, that can be used to analyze samples for the presence of Influenza A virus, Influenza B virus, Respiratory Syncytial Virus A, and/or Respiratory Syncytial Virus B target nucleic acids.

Methods for detection of influenza in samples

There is disclosed a composition of an aqueous solution comprising, consisting or consisting essentially of a flap endonuclease, a bulking agent and an organic buffer, wherein the aqueous solution has an inorganic salt concentration of 5 mM or less and wherein the composition is substantially free of glycerol.

Dried compositions containing flap endonuclease

Provided herein are compositions, kits, and methods for detecting methicillin-resistant(MRSA) nucleic acids. In some embodiments, the compositions, kits, and methods can be used to detect one or more of type i, ii, iii, iv, v, vi, vii, viii, ix, xii, xiii, xiv, xv, or xxi SCCmec right extremity junction (MREJ) MRSA nucleic acids and one or more of mecA, mecC, and/or an additional-specific gene.

Compositions and methods for detecting

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San Diego, CA, United States of America

Matthias Jost