Diasys Diagnostic Systems Gmbh

Immundiagnostik Ag

Astellas Pharma Gmbh

Tron—translationale Onkologie an Der Universitátsmedizin Der Johannes Gutenberg—universität Mainz Gemeinnützige Gmbh

Universitätsmedizin Der Johannes Gutenberg-universitat Mainz

Matthias Grimmler

Ugur Sahin

Michael Koslowski

Stefanie Hubich

Christoph Hartmann

Pia Schu

Holger Müller

Alexandra Lein

Erwin Metzmann

Yoshifumi Watazu

Günther Gorka

Felix Walzer

Franz-Paul Armbruster

Tobias Becker

Laura Kreckel

Carolin Menzenbach

Detlef Thônges

Thomas Masetto

Detlef THÖNGES

Diana Barea Roldàn

A method for quantitatively determining the amount of acetaminophen in a sample with greater precision, greater sensitivity and fewer interactions and fewer spectral and chemical interferences with other compounds contained in the sample. The method includes acetaminophen being hydrolyzed and the resulting p-aminophenol being reacted with a compound of general formula (III): in the presence of an oxidant to form a compound of general formula (IV): wherein R1 and R2, independently of one another, are selected from H, —CH, and —OCH, R3 is —CHand R4 is a Calkyl moiety with a terminal sulfonate group, with the proviso that at least one of R1 and R2 is —OCHand/or R4 additionally has at least one OH substituent, and then the amount of the compound of general formula (IV) in the reaction mixture being photometrically determined.

Quantitative acetaminophen analytics

A method for measuring the presence of calprotectin (S100A8/A) heterodimer in a biological sample using a particle-enhanced turbidimetric immunoassay (PETIA) based on monoclonal antibodies. The method can be adapted on automated standard analyzers and provides a reliable clinical measurement of calprotectin in faecal samples and extracts. The method is comparable to commerical two-site sandwich ELISA. The disclosed method counters spontaneous agglutination caused by calcium ions and low-molecular weight calcium-binding S100 proteins as observed with conventional PETIAs. The method can be used for measuring the presence of human calprotectin in stool, urine, serum, plasma, synovial liquid and other body liquids. Metrological traceability and high commutability with conventional immunoassays (ELISA) has been shown despite of different measurement principles used.

Method for determination of members of the S100 family of calcium binding proteins by immunoturbidimetry

A method for determining the amount of glycated hemoglobin (HbA1c), in which&#x2014;if required&#x2014;the erythrocytes in a sample are hemolyzed, the haemoglobin that is then released&#x2014;if required&#x2014;is contacted with a proteolytic agent and the glycated hemoglobin degradation products obtained in this way or otherwise are quantified is disclosed. In order to provide such a process and reagents employable therein that has/have the property of sufficient stability of the chemical compounds that are essential to the reaction, the provision of the requisite proteolytic agent in the form of an inactivated protease is proposed, which is then only reactivated in situ. For the stabilization of the hemoglobin, which is unfolded at a very low pH in the range from 1 to 3, at least one suitable stabilizer should be present in the hemolysis solution, and, where a leuco dye is used in connection with the determination of the amount of HbA1c, it is proposed that the latter be stabilized with particular phosphine compounds and/or thio compounds.

Enzymatic determination of HbA1c

A method for determining the amount of glycated haemoglobin (HbA1c), in which&#x2014;if required&#x2014;the erythrocytes in a sample are haemolysed, the haemoglobin that is then released&#x2014;if required&#x2014;is contacted with a proteolytic agent and the glycated haemoglobin degradation products obtained in this way or otherwise are quantified is disclosed. In order to provide such a process and reagents employable therein that has/have the property of sufficient stability of the chemical compounds that are essential to the reaction, for the stabilization of the haemoglobin which is unfolded at a very low pH in the range from 1 to 3, at least one suitable stabilizer is present in the haemolysis solution. Where a leuco dye is used in connection with the determination of the amount of HbA1c, it is proposed that the latter be stabilized with particular phosphine compounds and/or thio compounds, and, in particular embodiments, the requisite proteolytic agent is to be provided in the form of an inactivated protease which is then only reactivated in situ.

Enzymatic determination of HBA1c

Growing community of inventors

Elz, Germany

Matthias Grimmler