Ecole Polytechnique Federale De Lausanne

Abionic Sa

Ecole Polytechnique Féderale De Lausanne (epfl)

Matthias Geissbuehler

Theo Lasser

Nicolas Durand

Marcel Leutenegger

Iwan Maerki

Stefan Geissbuehler

Claudio Dellagiacoma

A nanofluidic biosensor system () comprising a bottom substrate () and a top substrate () between which are defined an input lateral aperture (), a nanoslit () which contains at least one functionalized area () and an output lateral aperture () or an internal reservoir (), said biosensor system () being adapted to let a solution containing biomolecules () enter the input lateral aperture () and successively pass through said nanoslit () and said output lateral aperture () or internal reservoir (); said biosensor system () furthermore comprising a gas evacuation subsystem (-) which is located between said nanoslit () and the biosensor external environment.

Gas evacuation system for nanofluidic biosensor

The invention describes a method and a microscopy system for imaging and analyzing stochastically and independently blinking point-like emitters. A multiple-order cumulants analysis in conjunction with an established blinking model enables the extraction of super-resolved environment-related parameter maps, such as molecular state lifetimes, concentration and brightness distributions of the emitter. In addition, such parameter maps can be used to compensate for the non-linear brightness and blinking response of higher-order cumulant images&#x2014;used for example in Super-resolution Optical Fluctuation Imaging (SOFI)&#x2014;to generate a balanced image contrast. Structures that otherwise would be masked by brighter regions in the conventional cumulant image become samples using spectral cross-cumulants.

Cumulant microscopy

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Lausanne, Switzerland

Matthias Geissbuehler