Fraunhofer-gesellschaft Zur Foerderung Der Angewandten Forschung E.v.

Dow Agrosciences LLC

Matthias Buntru

Simon Vogel

Stefan Schillberg

Rainer Fischer

Krishna M Madduri

Janna Mai Armstrong

Audrey Jane Etter

Markus Sack

Daniel Blessing

Tanja Holland

The technology provided herein relates to novel promoter constructs, method and systems for an increase in the target protein yield and/or allowing the use of lower template concentration in the cell-free protein synthesis systems.

Promoter construct for cell-free protein synthesis

The technology provided herein relates to novel methods and systems for cell-free protein synthesis, in particular to cell-free protein synthesis systems suitable for protein synthesis before and after freezing for a short- or long-term storage, wherein said system comprises a cryoprotectant.

Cell-free protein synthesis system

The present disclosure pertains to novel cell cultivation and cell and/or cell-derived product production processes that have advantages over currently existing fermentation strategies. The processes and methods according to the present disclosure may be used for an efficient supply of highly viable and metabolically active eukaryotic cells for transient production platforms, as an alternative production process with advantages over currently applied processes (batch, fed-batch or perfusion strategies) and for generating metabolically highly active biomass for subsequent use for transient expression systems or infection by a virus or pseudovirus or in cell-free systems.

Fermentation systems

This disclosure concerns the systems, methods, and kits for the in vitro synthesis of biological macromolecules in a reaction utilizing cell lysates containing plastids, mitochondria and/or chloroplasts, wherein creatine phosphate and creatine kinase are not added to the reaction to provide artificial energy regeneration.

Eukaryotic cell-free protein expression system that does not require an artificial energy regeneration system

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Aachen, Germany

Matthias Buntru